Fig. 7

Heatmap of DEG from all analyses, species and time points. Genes were included only if they were differentially expressed in at least one species, line or timepoint, and were expressed in all species (49 genes were excluded that were not present or not expressed in some species). The data for D. yakuba at 50h is excluded due to technical problems. The colors of the heatmap indicate the (log2-transformed) median fold change of the three replicates. The fold change was calculated as the ratio of counts per million of parasitized to control samples. The distance matrix was calculated using Pearson correlation and clustered using “ward.D2”. Each gene was annotated with eight functional and three orthologous categories (right panel, where the presence is indicated by a black filled squares)