Fig. 5
From: Genome-wide survey of miRNAs and their evolutionary history in the ascidian, Halocynthia roretzi
RT-PCR validation of miRNAs. a RT-PCR results of some well-conserved miRNAs listed in Table 1. b RT-PCR validation of some potential miRNAs that were predicted de novo and by using C. robusta small RNA-Seq reads. c Ascidian or tunicate-specific miRNAs (miR-4034, 4029 and 4123). ‘ + ’ indicates PCR with reverse transcriptase; ‘-’ indicates PCR without reverse transcriptase. The expected size of the amplified fragment is ~40 bp (arrowheads) because the 19 bp universal primer is amplified together with ~20 bp miRNA-specific primers. Other bands would be non-specific bands