Fig. 4

Changes in relative MICs by deletion of genes. a The log₂-transformed relative MICs (CP and ENX) to the parent strain observed in acrR, marR, atpI, ompF, and glyXY gene deletion mutants. The error bars represent the standard deviation of MICs obtained from 3 independent cultures. The bar for the relative MIC of glyXY deletion strain to CP is invisible, since the value of log2-transformed relative MIC is zero with zero standard deviation. b Predicted and observed relative MICs (CP and ENX) in double deletion mutants. Double deletion mutants were constructed, in which all possible pairwise combinations of above 5 genes were disrupted. The predicted MICs in double deletion mutants were calculated by a sum of log₂-transformed relative MICs of corresponding two single-gene deletion mutants. The solid black line represents y = x for reference