Fig. 4

Predicted upstream regulators from IPA analysis of gene expression changes from fasted to 1DPF. a Venn diagram of all upstream regulatory molecules analyzed. b Heatmap of predicted activation z-scores for selected classes of upstream regulatory molecules. Green indicates predicted activation, blue indicates predicted inhibition, white indicates the regulator is not predicted to function in that organ, and grey indicates that the upstream regulator is predicted to have significant involvement but the activation state cannot be determined based on the gene expression data. Regulators shown in this heatmap were filtered by three conditions: 1) were present in at least three of the four organs, 2) are significantly predicted (p-value < 0.05), and 3) have activation z-scores greater than |1.5| in at least one organ. Biological drug, chemical, and microRNA categories were excluded from URM analyses