Fig. 2

Expression of the eGFP-Htt construct. Induction of the eGFP-Htt construct was quantified by fluorescence measurements in the microscopy images and through the use of a custom probe set spotted on the arrays. Expression of eGFP-Htt protein was obtained by calculating total measured fluorescence divided by the combined cell volume for each of the at least four images analysed per time point for control (a) and HD (b) cells. The solid line connects the median values of each time point. c The hybridisation sites for the microarray probes designed to detect the eGFP-Htt mRNA are indicated. For each eGFP-Htt probe, the fold change in expression level over the pre-induction state (0 h) was calculated and plotted for each sampled time point in control (d) and HD (e) cells. Probes with the reverse complementary (rc) sequence of the expression probes were used as negative controls