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Table 5 Open reading frame and transcript prediction analysis of sugarcane transcriptome sequence data

From: A survey of the complex transcriptome from the highly polyploid sugarcane genome using full-length isoform sequencing and de novo assembly from short read sequencing

ORF prediction PacBio transcript isoforms De novo transcript contigs
ORF containing transcripts 100,639 491,544   
Retained transcriptsa 96,114 355,453   
Min length 300 300   
Max length 8,142 9,501   
N50 1,158 738   
Evigene prediction PacBio transcript isoforms De novo transcript contigs Unigenes SoGI
Total transcripts 51,025 83,041 13,205 41,042
Main transcripts 25,012 56,766 13,205 32,013
Alternate transcripts 26,013 26,275 0 9,029
Ave length 1 K proteinsb 1,348 298 298 287
  1. atranscripts with Pfam and Viridiplantae hits. bAverage length (aa) of the largest 1.000 proteins