Complete sequence and comparative genomic analysis of eight native Pseudomonas syringae plasmids belonging to the pPT23A family

Table 2 Main characteristics of native plasmids sequenced in this study

P. syringae strains	Plasmid name	Plasmid size (bp)	copABCD	rulAB	Type IVSS	%GC content	n° of ORF’s	Hp (n°-%) ^a
pv. syringae
UMAF0081	pPs0081	61,606	+^c	+^c	IVA (virB genes) ^c	55.52	66	23–34.8
UMAF0170	pPs0170	64,559	+^c	+^c	IVA (virB genes)	54.90	75	27–36.0
UMAF0158	pPs0158	63,004	-^c	+	IVA (virB genes)	54.58	65	22–33.8
UMAF1029	pPs1029	63,013	-^c	+	IVA (virB genes)	54.59	65	22–33.8
6–9	pPs6-9	61,606	+^c	+	IVA (virB genes)	55.52	66	23–34.8
7B44	pPs7B44	71,783	+^c	+	IVA (virB genes)	54.97	83	27–32.5
pv. garcae
2708	pPg2708	72,672	-^c	+^b	IVA (virB genes)	53.47	83	31–37.3
pv. tabaci
0893–29	pPt0893-29	73,842	-^c	+^c	IVB (tra genes)	56.01	81	29–35.8

^a Hp, number and percentage of hypothetical protein (number of hypothetical proteins divided by total number of proteins) present in each plasmid
^b The rulA and rulB genes are located in different positions in the plasmid sequence and in different orientations
^c Phenotypic analyses performed previously of highlighted plasmids have demonstrated copper resistance (copABCD), UV radiation tolerance (rulAB), and conjugation transfer (virB genes) [28, 29, 43, 88, 45, 89]

ISSN: 1471-2164