Fig. 3

qRT-PCR of candidate genes in tick stages. Quantitative RT-PCR expression analysis of RNA from T. annulata infected ticks generated at Day 2, Day 6, Day 10 and Day 15 post-detachment, relative to T. annulata merozoite Day 8 (calibrator) for: Tams1 (TA17050); putative papain-family cysteine protease (TA10955); Pfs 48/45 6-cys domain encoding gene TA03640 and 6-Cys like gene TA20855. * above (positive) or below (negative) error bars denote degrees of significant difference (* p < 0.05, ** p < 0.01, *** p < 0.0001) between fold-change at a time-point relative to merozoite calibrator RNA. Expression is depicted on a log₂ scale