Table 1 Bioinformatic characteristics of 12 transmission blocking candidate (surface) antigen genes
T. annulata ID | Chr | Product | Annotation | Signal peptide | GPI anchor | TMD | Macro to mero FC | Macro to piro FC | Mero to piro FC | T. parva ID | dN/dS | Protein identity | Nucleotide identity |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
TA02580 | 3 | Hypothetical protein | – | ✓ | ✓ | 0 | 2.14 | 2.74 | 1.28 | TP03_0040 | 0.0732 | 28.07 | 46.00 |
TA02585 | 3 | Hypothetical protein | – | ✓ | ✓ | 0 | 1.32 | 2.34 | 1.77 | TP03_0039 | 0.0127 | 31.29 | 47.24 |
TA03640 * | 3 | Hypothetical protein | Sexual stage antigen (Pfam:PF07422) | ✓ | ✓ | 0 | 3.55 | 4.14 | 1.17 | TP03_0268 | 0.2529 | 64.17 | 73.18 |
TA03755 | 3 | Sporozoite surface antigen (SPAG) | P67 sporozoite (Pfam:PF05642) | ✓ | ✓ | 1 | 4.23 | 4.29 | 1.01 | TP03_0287 | 0.3260 | 49.85 | 63.60 |
TA10955 * | 4 | Putative papain-family cysteine protease | Cysteine-type peptidase activity (GO:0008234) | ✓ | ✓ | 0 | 3.42 | 4.61 | 1.35 | TP04_0598 | 0.0904 | 85.50 | 83.48 |
TA13810 | 2 | Putative ts-chitose type 23 kDa piroplasm surface-like protein | Orthologous to T. sergenti merozoite surface antigen | ✓ | ✓ | 1 | 2.55 | 1.97 | −1.29 | TP02_0551 | 0.1638 | 83.41 | 84.57 |
TA13825 | 2 | Hypothetical protein | – | ✓ | ✓ | 1 | 12.40 | 11.00 | −1.13 | TP02_0553 | 0.4276 | 49.76 | 64.30 |
TA16005 | 2 | Hypothetical protein | Domain of unknown function DUF529 (Pfam:PF04385) | ✓ | ✓ | 1 | 1.87 | 3.09 | 1.65 | TP02_0950 | 0.3317 | 62.59 | 75.61 |
TA16565 | 1 | Hypothetical protein | – | ✓ | ✓ | 0 | 1.60 | 2.27 | 1.41 | TP01_1144 | 0.2704 | 74.68 | 79.69 |
TA17050 * | 1 | Merozoite-piroplasm surface antigen Tams1 | Merozoite antigen (Pfam:PF02488) | ✓ | ✓ | 1 | 2.62 | 2.64 | 1.01 | TP01_1056 | 0.2751 | 72.86 | 77.38 |
TA17220 | 4 | Hypothetical protein | Domain of unknown function DUF529 (Pfam:PF04385) | ✓ | ✓ | 1 | 1.66 | 2.81 | 1.69 | TP04_0030 | 0.3037 | 51.59 | 68.65 |
TA20855 * | 1 | Hypothetical protein | Similarity to P. yoelii (SWALL:EAA20932) and P. falciparum (SWALL:Q8IE86) | ✓ | ✓ | 0 | 2.57 | 2.63 | 1.03 | TP01_0412 | 0.085 | 80.43 | 77.72 |