Table 1 Summary of TraDIS data obtained from sequencing six barcoded S. equi mutant libraries, generated with pGh9:ISS1
Library | Unique insertion sites in genes | Total reads in genes | Genes containing insertions (% of total genes) | Library saturation (insertion every n bp in genes) |
|---|---|---|---|---|
CA | 54,815 | 1,645,725 | 1,787 (87.6) | 35 |
TC | 51,827 | 2,162,710 | 1,804 (88.5) | 37 |
AG | 66,384 | 1,816,701 | 1,792 (87.9) | 29 |
AC | 35,592 | 3,290,822 | 1,797 (88.1) | 54 |
CT | 32,502 | 3,171,602 | 1,804 (88.5) | 59 |
GA | 44,761 | 2,650,678 | 1,815 (89) | 43 |
master | 208,531 | 14,825,797 | 1,935 (94.9) | 9 |