Fig. 3

Gene Expression post LPS treatment of Selected Differentially Methylated Genes in Young and Old Fibroblast Cultures. Expression of six genes more methylated in young cultures (a) PIK3R1, (b) FES, (c) TNFSF13, (d) NFATC1, (e) TCF7 and (f) RORA were measured in young and old fibroblasts (n = 6 per group) at 0, 2 and 8 h post LPS. Expression was measured by RT-qPCR and values are presented as dCT in comparison to β-actin. Fold change gene expression (Old > Young) is displayed in parentheses above each significantly different time points. All values are displayed as mean (+/− SEM). Significance was determined using a paired Student’s t Test at each time point post LPS treatment and *** = P < 0.001, ** = P < 0.01, * = P < 0.05, † = P < 0.10