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Fig. 7 | BMC Genomics

Fig. 7

From: Application of nonsense-mediated primer exclusion (NOPE) for preparation of unique molecular barcoded libraries

Fig. 7

HTS data analysis. EGFR-ex20 gene libraries were prepared in parallel using NOPE and ExoI approaches for EGFR-ex20_NNN primer exclusion. a The number of detected UMI sequences with a sufficient (5+ read) coverage. b UMI coverage distribution showing the fraction of reads (Y axis) tagged with UMIs covered X times. Dashed line shows reads-per-UMI threshold selected for data analysis. c Identification of T790 M mutation in EGFR gene in control PBMC DNA, the reference standard and their 9:1 mixture. Red and blue lines show the expected mutation frequency in the mixture and pure reference standard sample, respectively

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