Fig. 6

Differential usage of exons detected by RNA-seq and HTA show low similarity. The analysis was based on exon (a) or junction (b) expression with FDR < 0.05 and |logFC| ≥ log₂(1.5). The intersection of exons confirmed by both approaches within RNA-seq and HTA platforms is shown (c). The exon parameters distribution among differentially used exons detected by the two platforms is also show in (d,e). The relative position of the exons within their genes, varying from 5′ end (relative position = 0) to 3′ end (relative position = 1), shows a 3′ bias in RNA-seq (d). Exon length shows that RNA-seq tends to find more significantly splice events among long exons than HTA (e)