Fig. 7

Glycogen and trehalose accumulation in the wild-type and Δpac-3 mutant strains at 10 and 300 mM of CaCl₂. Mycelial samples from the wild-type and Δpac-3 mutant strain cultured in the absence of Ca²⁺ (zero) at 30 °C for 24 h and shifted to calcium stress (10 mM or 300 mM of CaCl₂) for 15, 30 and 60 min were used for glycogen and trehalose quantification. The asterisks indicate significant differences compared to the zero sample in the wild-type or mutant strains (Student’s t-test, P < 0.01). All results represent the average of at least four independent experiments. Bars indicate the standard deviation from the biological experiments