Fig. 5
From: Relatively frequent switching of transcription start sites during cerebellar development
Alternative TSSs in glypican 6 (Gpc6) and experimental validation of its non-crossover switching events with Real-time PCR. a Schematic DNA structure of Gpc6, alternative mRNA variants and un-altered protein structure. b in situ expression of Gpc6 in mouse cerebellum at E14.5 (from GenePaint). c HeliscopeCAGE expression data for the two alternative TSSs during cerebellar development. X-axis: time, from embryonic day 11 (E11) to postnatal day 9 (P9). Y-axis: expression level measured in tpm (tags per million). d qRT-PCR expression data demonstrating a non-crossover TSS switching event between E15 and P9. X-axis: time at E12, E15 and P9. Y-axis: expression level measured in RQ (relative quantity against H2O as negative control)