Brassica napus infection rates and global transcriptome changes with combinations of PA23 and Sclerotinia sclerotiorum.
a Numbers of lesion-forming petals as a percentage of total petals which fell onto plant leaves in greenhouse assays. b
S. sclerotiorum disease progression on canola leaves at 24 h or 48 h after petal application. PA23 + Ss treatment petals were inoculated with PA23 24 h prior to S. sclerotiorum inoculation, whereas Ss treatment petals were inoculated with sterile water. Petals from both treatment groups (PA23 + Ss and Ss) were then infected in vitro with S. sclerotiorum 48 h prior to being placed on leaves. c Principal component analysis of mRNA sequences from the four treatment groups examined by RNA-seq. Variation between treatments is greater that variation between replicates, and phenotypically similar treatment groups clustered more closely together. d Number of unique mRNAs present in treatment groups, as well as the cumulative number of unique mRNA transcripts identified. Transcripts are categorized by frequency of occurrence in the library, as described by the number of fragments per kilobase of transcript per million mapped reads (FPKM) value