Fig. 5
Changes detected in the Brassica napus chloroplast in response to combinations of PA23 and Sclerotinia sclerotiorum. a Differential expression of genes associated with chloroplast-related GO terms, compared to the water control. Only genes with a log2 fold change greater than 2 in at least one treatment group are shown. I, chlorophyll catabolite transmembrane transport; II, chloroplast inner membrane; III, chloroplast photosystem I & II; IV, chloroplast stroma; V, chloroplast thylakoid; VI, plastoglobule; VII, protein import into chloroplast stroma; VIII, thylakoid lumen; IX, thylakoid membrane. b-f Transmission electron micrographs of leaf chloroplasts. b Water control, 48 h. c PA23, 24 h. d PA23, 48 h. e PA23 + Ss, 24 h. f Ss, 48 h. S = starch granule; g = grana stack; M = mitochondria. Arrows indicate plastoglobules. Scale bar for panels A-E = 500 nm. g Chlorophyll b content of treated leaves. h Relative abundance of the chlorophyllase 1 transcript as determined by RNA-seq (grey bars) and qRT-PCR (black line)