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Fig. 3 | BMC Genomics

Fig. 3

From: Systematic discovery of novel eukaryotic transcriptional regulators using sequence homology independent prediction

Fig. 3

Experimental analysis of the predictions. a Steps of the in planta transactivation assay procedure from bacterial growth to quantification of the normalized transactivation activity. b Constructs used in the transactivation assay. c Average relative transactivation activity calculated as the GUS activity (nmol of 4MU/min/mg total protein) divided by the concentration of the effector protein (ng/ml). Error bars represent standard error from 3 independent experiments. The asterisk (*) indicates that the relative activity is statistically different from the YFP control (p-value <0.002, t-test). A line under the gene names indicates that they belong to the same family

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