Table 4 Main proteins involved in biomass deconstruction regulation and their expression in A. niger N402 and T. reesei RUT-C30 transcriptomes
Gene | ID A. niger | ID T. reesei | A. niger up regulation (Log2(FC)) | T. reesei up regulation (Log2(FC)) | Function | References |
|---|---|---|---|---|---|---|
xlnR/xyr1 | An15g05810 | 98788 | 6h (2.59), 12h (2.27), 24h (2.22) | 24h (1.65) | Major activator for cellulase and xylanase genes | |
creA/cre1 | An02g03830 | 23706 | 6h (1.18), 12h (1.50) | - | Key regulator of CCR, suppressing the transcription of cellulase and xylanases genes | |
araR | An04g08600 | Â | - | - | Control the L-arabinose catabolic pathway and the expression of genes encoding to enzymes related to arabinose metabolism | [127] |
amyR | An04g06910 | 42772 | - | - | Regulator of starch degradation and controls the production of D-glucose and D-galactose releasing enzymes | [128] |
ace1 | An16g02040 | 122363 | - | - | Repressor of cellulase and xylanase gene expression | [129] |
ace2 | - | 32395 | - | - | Activator of cellulase and xylanase gene expression | [130] |
ace3 | - | 98455 | - | 12h (1.27), 24h (2.11) | Activator of cellulase and xylanse gene expression and has an unclear role on xyr1 expression | [131] |
inuR | An15g00300 | - | 24h (1.42) | - | Induce the expression of inulinolytic genes | [80] |
bglR | - | 91236 | - | - | Activator of specific β-glucosidase genes expression | [132] |
clr-1 | An05g00020 | 68701 | - | - | The orthologue in Neurospora crassa has positive role on cellulase production | [133] |
clr-2/clrB/ manR | An12g01870 | 76250 | - | 6h (2.13), 12h (2.87), 24h (3.40) | The orthologue in Neurospora crassa has positive role on cellulase production. In A. oryzae, is a regulator of genes enconding mannan and cellulose degrading enzymes | |
galX | An16g01640 | - | 24h (1.11) | - | Regulates the D-galactose oxido-reductive pathway | [135] |
galR | - | - | Â | - | Regulates the D-galactose oxido-reductive pathway in A. nidulans | [72] |
hap-2/hapB | An15g03650 | 93466 | - | - | Form the protein complex Hap2/3/5 that represents a positive transcriptional activator that binds to the promoter region of cellobiohydrolase gene | [136] |
hap-3/hapC | An01g02620 | 24298 | - | - | Form the protein complex Hap2/3/5 that represents a positive transcriptional activator that binds to the promoter region of cellobiohydrolase gene | [136] |
hap-5/hapE | - | 125434 | - | - | Form the protein complex Hap2/3/5 that represents a positive transcriptional activator that binds to the promoter region of cellobiohydrolase gene | [136] |
rhaR | An13g00910 | 101004 | 6h (1.75), 12h (1.18), 24h (1.58) | - | Activator of pectinase-encoding genes | [77] |
malR | An11g04160 | 133808 | 6h (2.41), 12h (2.20), 24h (2.02) | 6h (1.17), 12h (1.54) | Controls the expression of maltose utilizing genes | [73] |
xpp1 | Â | 101064 | - | - | Regulator of xylanases but not cellulases genes | [137] |
ron1/xprG | An16g09130 | 112541 | 6h (1.24) | - | Key activator of the GlcNAc gene cluster and essential for chitin catabolism | [138] |
clbR | An12g04970 | - | - | - | Regulator involved in cellobiose and cellulose induction | [139] |
pacC/pac1 | An02g07890 | 95791 | - | 6h (2.28), 12h (2.15), 24h (1.94) | pH transcription regulator, also involved in cellulose gene expression | |
areA | An12g08960 | 140814 | - | - | A global nitrogen metabolism regulator, with influence on cellulase production | [142] |