Fig. 2

Overall characteristics of AS events. a The proportions for the three types of dinucleotides at the splicing border. b Number and frequency of the different AS types. (IR) Intron retention, (A3SS) alternative 3′ splice site, (A5SS) alternative 5′ splice site, (ES) exon skipping. c Four genes representing four types of AS events were validated by RT-PCR. The lanes from left to right are DNA ladder, leaf and root of ‘NAU-RG’, ‘NAU-LB’, ‘NAU-YH’, and genomic DNA of ‘NAU-YH’. The RNA-Seq read density of the four genes was viewed by IGV browser (green peaks). The red arrows indicate alternative splice sites. The blue arcs indicate splice junction reads that support the junctions