Table 3 Descriptive statistics of novel Passiflora edulis microsatellite markers
# | Marker | Primer Sequences 5′-3′ | RepeatMotif | Ta | AlleleN | Allele Size Range (bp) | He | Ho | PIC |
|---|---|---|---|---|---|---|---|---|---|
1 | BrPe0032 | F:TTGCACAATGACCAATGTTGT R:CTGAGCACCTTGTCAAAATACA | (AT)13 | 60 °C | 7 | 137–157 | 0.84 | 1.00 | 0.77 |
2 | BrPe0028 | F:CAAAAGGAACAGGGAAGA R:GAAAGAGAGAAAGACAGAGA | (TA)6 | 55 °C | 5 | 90–110 | 0.80 | 0.50 | 0.72 |
3 | BrPe0024 | F:CCCTACCTTTCTCTGCTT R:CATCTCCTCTATCTCCTTC | (TC)7 | 55 °C | 4 | 221–231 | 0.76 | 0.70 | 0.68 |
4 | BrPe0031 | F:AGGTCGGTGGGTGTGTTTAG R:CATTCAACTCCCCAAAAGGT | (TA)9 | 60 °C | 5 | 134–150 | 0.77 | 0.67 | 0.67 |
5 | BrPe0014 | F:AATATGGCTGGGGAAAAC R:TTCCTGTCTTTGGACCTT | (AG)7 | 57 °C | 5 | 215–227 | 0.75 | 0.50 | 0.67 |
6 | BrPe0033 | F:GCCATGAGAGACTTGGGAGA R:CGGTTGCCAAAAAGAAGAGA | (AT)8 | 60 °C | 5 | 237–249 | 0.72 | 0.30 | 0.65 |
7 | BrPe0038 | F:TTTCAACTTTTCGTGTGTGC R:TGTTGTTGCTTGGAAGGATG | (AT)6 | 60 °C | 5 | 154–176 | 0.73 | 0.60 | 0.64 |
8 | BrPe0042 | F:CATGCATTCATTTGTTTTTCTTG R:GATGCTGGGAAAAAGAGTGC | (AT)8 | 60 °C | 6 | 142–160 | 0.71 | 0.80 | 0.63 |
9 | BrPe0003 | F:CTTTCTCTCCCTATACCC R:CCCTCCATAATCACATAAC | (TC)11 | 55 °C | 5 | 277–291 | 0.70 | 0.40 | 0.62 |
10 | BrPe0043 | F:TCATACATGGATGTCAAATCGATAC R:GCGGACCAAGAAAATTCAAA | (AT)8 | 60 °C | 4 | 199–207 | 0.71 | 0.50 | 0.60 |
11 | BrPe0006 | F:AAGGAAAAGAACAGCCTCA R:CGCTCTCAAATCAGTCAAA | (TC)10 | 55 °C | 4 | 193–199 | 0.68 | 0.40 | 0.59 |
12 | BrPe0002 | F:AAAGCCCAGATGAAGTGAA R:GGCTCCAATCAGAAGTGT | (AG)12 | 55 °C | 3 | 177–185 | 0.69 | 0.56 | 0.58 |
13 | BrPe0021 | F:ACTTCCTCATCATTCG R:GCTATGCCTCTTTTTG | (TA)7 | 55 °C | 3 | 158–164 | 0.67 | 0.25 | 0.56 |
14 | BrPe0036 | F:TCGGACCTTAAAACCGAGAA R:CAGCACCAAAATTTGACGAG | (TC)6 | 60 °C | 4 | 197–203 | 0.65 | 0.10 | 0.54 |
15 | BrPe0023 | F:AGATACCACACCCAATAG R:TTGGAGTTGTTGGGGA | (CT)7 | 55 °C | 6 | 118–132 | 0.57 | 0.40 | 0.52 |
16 | BrPe3011 | F:CCGGTCTTCCTGATTGACTC R:CCTCTCTCACCTGGAACTGC | (TTC)4 | 60 °C | 3 | 157–163 | 0.62 | 0.30 | 0.50 |
17 | BrPe0037 | F:TGATAATGCAGCGAAAGAGC R:TCACACTCCATTTGCTCTGC | (TG)6 | 60 °C | 3 | 227–231 | 0.61 | 0.20 | 0.49 |
18 | BrPe0010 | F:GAAGAAAAAAGGGCTTG R:GTTAGGGTTTGGAGGA | (TC)9 | 55 °C | 3 | 200–204 | 0.60 | 0.40 | 0.48 |
19 | BrPe0001 | F:GTTGAGAGGATTGTGTTTG R:ATGGTAGAGGAGGAGAGA | (CT)14 | 55 °C | 3 | 143–157 | 0.56 | 0.14 | 0.46 |
20 | BrPe0012 | F:AGAGAGAGAGAGAGAG R:ACATCATACTCCTCATCC | (AG)8 | 55 °C | 3 | 214–218 | 0.58 | 0.13 | 0.45 |
21 | BrPe0008 | F:TTTTCAGCCTCCACTCTT R:TACACCACCAACACTCAC | (AG)9 | 55 °C | 3 | 264–274 | 0.57 | 1.00 | 0.44 |
22 | BrPe0025 | F:CAAGGAACCAGAACAAGAAGAA R:GAAGAACAAGCCAGCCCA | (GA)6 | 55 °C | 3 | 114–126 | 0.57 | 0.11 | 0.44 |
23 | BrPe0039 | F:GCTGCTCCACTGTGAATGTC R:AACCTAGCCCCGTCACAGTA | (AT)6 | 60 | 3 | 193–203 | 0.57 | 0.10 | 0.44 |
24 | BrPe0050 | F:TCAAGGGTATCTTTGGTGCTG R:AGCTTCAGCGAGACAAAACC | (TG)7 | 60 °C | 3 | 197–205 | 0.56 | 0.20 | 0.44 |
25 | BrPe0013 | F:GATCGAGGTGAGGTACTG R:GGTTTGGCTTTAATGGAGG | (AG)8 | 55 °C | 2 | 169–171 | 0.53 | 0.00 | 0.38 |
26 | BrPe0020 | F:TAAAGCATCAGGTCAG R:TAGATAGATTTGACGGG | (GT)7 | 55 °C | 2 | 295–297 | 0.53 | 0.00 | 0.38 |
27 | BrPe0034 | F:CCTGTGGTGAAAATGGAACC R:GAGCCCTGGACTGACACATT | (CT)15 | 60 °C | 2 | 217–227 | 0.56 | 1.00 | 0.38 |
28 | BrPe0049 | F:GGGAATCAAAACCATGCAGT R:CTCCCAGCTTCCACTCACTC | (TA)9 | 60 °C | 2 | 189–191 | 0.53 | 0.11 | 0.38 |
29 | BrPe3012 | F:CGCCCTTTCTGAAGATAATCC R:GCAATGCTAAGAAGGCCAAG | (TCT)4 | 60 °C | 2 | 181–183 | 0.53 | 0.11 | 0.38 |
30 | BrPe0018 | F:TCCTTCCTTCTCCTCC R:ACACTTGTCTCTCATCT | (CT)7 | 55 °C | 3 | 135–149 | 0.43 | 0.30 | 0.37 |
31 | BrPe0022 | F:GGCATAGAAGTGGAAGGG R:GGAAGGGAAGTGAAGGGA | (AG)7 | 55 °C | 2 | 98–104 | 0.51 | 0.20 | 0.37 |
32 | BrPe0047 | F:TGGGCCATTTCTTTTCTCTC R:GAATCCTGCATGAGTTGAGGA | (CT)9 | 60 °C | 2 | 186–192 | 0.48 | 0.30 | 0.35 |
33 | BrPe3014 | F:CGGAAGCGTGCTCATAAAGT R:AAGCCTGTGAGGTTGATTCG | (AGA)5 | 60 °C | 2 | 218–220 | 0.48 | 0.30 | 0.35 |
34 | BrPe0007 | F:AAAGCCCAGATGAAGTGAA R:GGCTCCAATCAGAAGTGT | (AG)9 | 55 °C | 2 | 177–179 | 0.40 | 0.50 | 0.31 |
35 | BrPe0027 | F:TCCAATCTTCTCAACC R:CAAACTAGTAAACCCC | (TA)6 | 46 °C | 3 | 97–101 | 0.35 | 0.20 | 0.30 |
36 | BrPe3027 | F:CCAAAATGCCCAAAATGTCT R:GTCCGTGAGGAGATGTCGAT | (GGT)4 | 60 °C | 3 | 178–202 | 0.35 | 0.40 | 0.30 |
37 | BrPe0019 | F:AAAGAGAAGGATGGATG R:AAAAAGGACGAGGAAGA | (TC)7 | 55 °C | 2 | 210–214 | 0.36 | 0.14 | 0.28 |
38 | BrPe0044 | F:GGACGCTAAGAGACCCATTG R:TAAAAGCCCCACTTGCAATC | (TA)6 | 60 °C | 2 | 217–219 | 0.33 | 0.38 | 0.26 |
39 | BrPe0016 | F:TGGTTGGTGGGTCTTGT R:CTCTTTCCTCTCTCTCTCTCT | (AG)7 | 55 °C | 2 | 277–279 | 0.21 | 0.22 | 0.18 |
40 | BrPe0045 | F:CGCTTCCACTTTACCAGCTC R:GACCAACAACAGGCACAATG | (GT)8 | 60 °C | 2 | 183–185 | 0.21 | 0.22 | 0.18 |
41 | BrPe0011 | F:GTTCTACTCCCTCATT R:CTTCTTAACATCCCCA | (CT)8 | 53 °C | 2 | 74–80 | 0.19 | 0.20 | 0.16 |
42 | BrPe0017 | F:TTGTCTCTCGGTTCTCT R:CAAACACAAAACCCCC | (AG)7 | 55 °C | 2 | 86–90 | 0.19 | 0.00 | 0.16 |
| Â | AVERAGE | Â | Â | Â | 3.26 | Â | 0.55 | 0.35 | 0.45 |