Fig. 1

Shortening of telomeres and loss of viability during passaging of H.polymorpha D_TER strain. a Cell viability test for clones #1 and #2. Viability is expressed as colony forming units (CFU) per unit of OD₆₀₀. b Southern blot analysis of telomere-proximal restriction fragments in the wild-type and D_TER strains (clones #1 and #2). The position of DNA marker is shown on the left. Tel – positions of shortest telomere-proximal fragments. Note that clone #1 (passages 4 and 5) and of clone #2 (passage 5) represents telomerase-independent “survivals”