Fig. 3
Analysis of apurinic/apyrimidinic (AP) endonuclease activity in wild-type and D_TER strains. a PAGE analysis of 5′-[32P]-labelled AP site-containing DNA duplex upon incubation with protein extracts from wild-type and D_TER strains. The reaction mixtures were incubated at 37 °C for 30, 60 and 90 s. Processing of AP site resulted in the appearance of ~15 nt band. The nucleotide sequence of the target DNA duplex is shown in the upper part. b Quantitative analysis of the results is shown in panel A. The cleavage efficiency was defined as the percentage (%) of 15-nt product formation, which was calculated as the ratio of 15-nt products to the sum of all products identified on the gel. The values represent the average of three independent measurements