Table 1 H. walsbyi HBSQ001 general RNA sequencing statistics

Chromosome length (nt)

Total number of PE100 reads mapped to chromosome

111,441,003

115,537,266

Reads mapped to ribosomal operons

88,414,038

90,353,332

Reads mapped to CDS

3,272,328

3,925,912

Percent reads mapped to CDS

2.9%

3.4%

Percent reads mapped to ribosomal operons

79.3%

78.2%

Percent reads mapped to Intergenic regions

16.5%

17.2%

Percent reads mapped to UTR’s

15.1%

15.2%

Percent reads mapped to non UTR intergenic region

1.5%

2.0%

Percent reads mapped to SRP RNA

0.31%

0.28%

Percent reads mapped to RNAseP

0.88%

0.95%

Average coverage per nucleotide

199×

245×

Median number of reads per CDS

370

430

Average number of reads per CDS

1155

1386

Median TPM per CDS

8.8

9.9

Average TPM per CDS

21.3

23.4

Percentage of CDS expressed (>Percentile 10 (less than 37 and 43 reads)

17.6%

17.2%

Percentage of CDS expressed (>0 reads) (6 CDS)

94.6%

94.9%

GC

55%

54%