Fig. 1

Effect of treatment on gene expression. a The RIL populations were exposed to three treatments: control (48 h at 20 °C), heat stress (46 h at 20 °C and 2 h at 35 °C), and recovery (as heat stress, with an additional 2 h at 20 °C). At the end of these treatments, the nematodes were in the L4 stage, and were harvested. Thereafter RNA was isolated and the transcriptome was measured by microarray. b The outcome of the treatment analysis. On the left a legend is included to clarify which contrasts are compared. On the right, the overlap in differentially expressed genes is shown per treatment comparison. For example, 942 genes are uniquely differentially expressed in the recovery treatment, these genes are differently expressed between recovery and heat stress and between recovery and control, but not between heat stress and control