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Table 4 Gene copy number predictions and validations for a CRP3710 subfamily

From: Hybrid assembly with long and short reads improves discovery of gene family expansions

accession: HM101 HM034 HM056 HM340
A. Assembly
 MT4.0 2    
 ALLPATHS   1 3 3
 PBJelly   8 5 3
 Alpaca   29 26 30
B. Coverage (RPM)
 Medtr1g061160 (control 1) 0.26 0.38 0.29 0.50
 Medtr1g080770 (control 2) 0.29 0.59 0.51 0.57
 CRP3710 7.00 5.60 9.00 14.00
 estimated copy number 25.5 11.5 22.5 26.2
C. qPCR
 estimated copy number 12.0 11.0 9.7 8.9
  1. A. Annotation found between 1 and 30 copies per assembly. B. Coverages in reads per million bases for this gene and two controls, followed by the copy numbers estimated by fold increase of gene over control average, per accession. C. Copy numbers estimated from quantitative PCR per accession