Comparative analysis of targeted long read sequencing approaches for characterization of a plant’s immune receptor repertoire

Table 1 Comparison of ONT MinION R7.3 and PacBio RSII sequencing performance values: MinION fail and pass and PacBio RSII SR and RoI amount of reads, read quality and read size

	MinION fail Template	MinION fail Complement	MinION fail 2D	MinION pass Template	MinION pass Complement	MinION pass 2D	PacBio Subreads	PacBio Reads of Insert
Number of reads [n]	268,044	112,405	83,692	193,850	193,850	193,850	383,981	101,331
Number of bases [Mbp]	630	273	209	507	484	503	1360	353
Modal accuracy	74.88%	60.19%	84.15%	74.88%	74.88%	92.06%	90.00%	99.99%
Mean accuracy	70.24%	66.42%	82.62%	77.84%	76.79%	91.36%	89.83%	99.57%
N50 reads length [bp]	2916	2829	2786	2278	2169	2262	3540	3559
Mean read length [bp]	11,665	10,121	3250	2838	2716	2813	3818	3675
Modal read length [bp]	1138	2454	2306	2570	2720	2586	3482	3485

ISSN: 1471-2164