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Table 2 Read statistics after adapter trimming, chimera filtering and correction: Adapter trimming, removal of reads smaller than 150 bp and chimera filtering reduced the number of Mbp in each dataset by approximately 5%. Due to the lower quality MinION reads were Canu corrected before assembly. The Canu pipeline further reduced the amont of MinION reads to 304 Mbp before assembly – a number similar to the amount of PacBio RoI

From: Comparative analysis of targeted long read sequencing approaches for characterization of a plant’s immune receptor repertoire

 

MinION 2D pass (trimmed, filtered)

MinION 2D pass (trimmed, filtered, corrected)

PacBio Reads of Insert (trimmed, filtered)

Number of reads [n]

193,724

114,027

100,958

Number of bases [Mbp]

475

304

337

N50 reads length [bp]

2681

2739

3430

Mean read length [bp]

2681

2784

3536