Fig. 6

MiRNA cluster comparison between fish. a Amount of clusters and their respective sizes with a maximum distance of 10,000 bp between two miRNAs. (nfu - Nothobranchius furzeri, dre - Danio rerio, ola - Oryzias latipes, gac - Gasterosteus aculeatus, tru - Takifugu rubripes). b Structure comparison of the miR-17/92 cluster. Two highly conserved clusters could be identified for each species, as well as some smaller less conserved clusters, containing at least two miRNAs of the miR-17/92 cluster. c Structure comparison of the miR-430 cluster. No structural similarity between the different species can be observed. However, D. rerio, G. aculeatus and N. furzeri show some distinct but individual repeating pattern. Even though the gene variants miR-430b and miR-430d seem to be unique to D. rerio and O. latipes, they can be clearly distinguished, based on sequence alignments. d After the ancestral duplication event, the mir-29 cluster is distinguished in the mir-29a/b-1 (filled red and blue dots) and the mir-29a/b-2 cluster (red and blue circles). Whereas for D. rerio the mir-29a-2 gene seems to be lost, we assume that for G. aculeatus the whole second mir-29 cluster (dashed circles) is only missing, because of the low quality genome sequencing and assembly. In N. furzeri we observe an additional copy for each of the two clusters, except that the mir-29a/b-1 pair was only partially duplicated or the second mir-29a-1 gene was lost again