Table 3 Ranking of different candidate reference genes as determined by 4 different algorithms delta CT. BestKepper, NormFinder, geNorm
a. Ovary | ||||||||||
Ranking Order (Better--Good--Average) | ||||||||||
 Method | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
 Delta CT | eef1 | g6pd | tubulin | b2mg | 18S rRNA | tbp | gapdh | βactin | rbpl13 | ubi |
 BestKeeper | eef1 | tubulin | b2mg | tbp | g6pd | 18S rRNA | gapdh | βactin | rbpl13 | ubi |
 Normfinder | eef1 | g6pd | 18S rRNA | tubulin | b2mg | tbp | gapdh | βactin | rbpl13 | ubi |
 geNorm | eef1 | tubulin | b2mg | tbp | g6pd | 18S rRNA | gapdh | βactin | rbpl13 | ubi |
 Recommended comprehensive ranking | eef1 | tubulin | G6PD | b2mg | 18S rRNA | tbp | gapdh | βactin | rbpl13 | ubi |
b. Testis | ||||||||||
Ranking Order (Better--Good--Average) | ||||||||||
 Method | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
 Delta CT | b2mg | tbp | ßactin | g6pd | eef1 | 18S rRNA | gapdh | rbpl13 | tubulin | ubi |
 BestKeeper | b2mg | tbp | g6pd | eef1 | tubulin | gapdh | ßactin | rbpl13 | 18S rRNA | ubi |
 Normfinder | b2mg | tbp | ßactin | g6pd | 18S rRNA | eef1 | gapdh | rbpl13 | tubulin | ubi |
 genorm | tbp | eef1 | b2mg | g6pd | ßactin | gapdh | 18S rRNA | rbpl13 | tubulin | ubi | |
 Recommended comprehensive ranking | b2mg | tbp | eef1 | g6pd | ßactin | gapdh | 18S rRNA | tubulin | rbpl13 | ubi |