Gene symbol | Primer Forward | Primer Reverse | Amplicon Size | Tm | Fish species | Accession no. | PCR Efficiency (%) | Reference |
---|---|---|---|---|---|---|---|---|
18S rRNA | GGTAGACACACGCTGATCCA | CATGGCCGTTCTTAGTTGGT | 96 | 60 | Channa striatus | KC800799 | 99.1 | Purohit et al. 2015 [10] |
βactin | GCCTTCCTTCCTTGGTATGG | GTGTTGGCGTACAGGTCCTT | 89 | 59 | Channa striatus | KC967219 | 98.50 | Purohit et al. 2015 [10] |
b2mg | GCCTTCACCCCAGAGAAAGG | GCGGTTGGGATTTACATGTTG | 103 | 55 | Danio rerio | BC062841 | 99.97 | McCurley and Callard 2008 [13] |
tub | CCTGCTGGGAACTGTATTG | TCAATGAGTTCCTTGCCAAT | 89 | 54 | Channa striatus | KC710731 | 97.64 | Purohit et al. 2015 [10] |
eef1 | CTTCTCAGGCTGACTGTGC | CCGCTAGCATTACCCTCC | 109 | 56 | Danio rerio | AY422992 | 99.86 | McCurley and Callard 2008 [13] |
gapdh | ATCAAGGAAGCGGTGAAGAAGG | CGAAGATGGAGGAGTGGGTGTC | 99 | 60 | Channa striatus | KF044311 | 95.21 | Purohit et al. 2015 [10] |
g6pd | GTCCCGAAAGGCTCCACTC | CCTCCGCTTTCCTCTC | 112 | 62 | Danio rerio | BMI82602 | 98.65 | McCurley and Callard 2008 [13] |
rbpl1 | ATGAACACCAACCCTTCCCG | GGTGGAGGGATGCCATCAAA | 95 | 59 | Channa striatus | KC915026 | 96.88 | Purohit et al. 2015 [10] |
tbp | CTTACCCACCAGCAGTTTAGCAG | CCTTGGCACCTGTGAGTACGATTTG | 126 | 57 | Channa striatus | KF736933 | 95.37 | Purohit et al. 2015 [10] |
ubi | GGCCGCAAAGATGCAGAT | CTGGGCTCGACCTCAAGAGT | 69 | 58 | Gadus morhua | EX735613 | 99.78 | Olsvik et al. 2008 [35] |