Suitable reference gene for quantitative real-time PCR analysis of gene expression in gonadal tissues of minnow Puntius sophore under high-temperature stress

Table 4 Candidate genes, primer pairs and different q-PCR parameters

Gene symbol	Primer Forward	Primer Reverse	Amplicon Size	Tm	Fish species	Accession no.	PCR Efficiency (%)	Reference
18S rRNA	GGTAGACACACGCTGATCCA	CATGGCCGTTCTTAGTTGGT	96	60	Channa striatus	KC800799	99.1	Purohit et al. 2015 [10]
βactin	GCCTTCCTTCCTTGGTATGG	GTGTTGGCGTACAGGTCCTT	89	59	Channa striatus	KC967219	98.50	Purohit et al. 2015 [10]
b2mg	GCCTTCACCCCAGAGAAAGG	GCGGTTGGGATTTACATGTTG	103	55	Danio rerio	BC062841	99.97	McCurley and Callard 2008 [13]
tub	CCTGCTGGGAACTGTATTG	TCAATGAGTTCCTTGCCAAT	89	54	Channa striatus	KC710731	97.64	Purohit et al. 2015 [10]
eef1	CTTCTCAGGCTGACTGTGC	CCGCTAGCATTACCCTCC	109	56	Danio rerio	AY422992	99.86	McCurley and Callard 2008 [13]
gapdh	ATCAAGGAAGCGGTGAAGAAGG	CGAAGATGGAGGAGTGGGTGTC	99	60	Channa striatus	KF044311	95.21	Purohit et al. 2015 [10]
g6pd	GTCCCGAAAGGCTCCACTC	CCTCCGCTTTCCTCTC	112	62	Danio rerio	BMI82602	98.65	McCurley and Callard 2008 [13]
rbpl1	ATGAACACCAACCCTTCCCG	GGTGGAGGGATGCCATCAAA	95	59	Channa striatus	KC915026	96.88	Purohit et al. 2015 [10]
tbp	CTTACCCACCAGCAGTTTAGCAG	CCTTGGCACCTGTGAGTACGATTTG	126	57	Channa striatus	KF736933	95.37	Purohit et al. 2015 [10]
ubi	GGCCGCAAAGATGCAGAT	CTGGGCTCGACCTCAAGAGT	69	58	Gadus morhua	EX735613	99.78	Olsvik et al. 2008 [35]

ISSN: 1471-2164