Fig. 7

Inhibition of IFN signalling in L929 cells using siRNAs targeted to murine Ifnar1 (siIFNAR1). a Expression of Ifnar1 following treatment with siIFNAR1. b Average number of nuclei per syncytium in siIFNAR treated and non-specific siRNA treated cells infected with NBV at 24 and 40 hpi. c Visualisation by immunofluorescence microscopy of siIFNAR1 treated and non-specific siRNA treated cells following infection with NBV at MOI 10 at 24 and 40 hpi. (DAPI in blue and NBV σ proteins in green). Representative pictures are presented here. *p < 0.05, ***p < 0.001, ns = non-significant. d NBV titre following infection at MOI 10 in siIFNAR1+ and non-specific siRNA L929 cells (error bars showing standard error from the mean, n = 3)