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Table 1 Relative expression values of genes during colonization of autoclaved sclerotia of Sclerotinia sclerotiorum by Aspergillus aculeatus Asp-4

From: Characterization of mechanisms underlying degradation of sclerotia of Sclerotinia sclerotiorum by Aspergillus aculeatus Asp-4 using a combined qRT-PCR and proteomic approach

Putative function

Gene ID

Time after inoculation (Mean +/− SD)

  

12 h

24 h

36 h

48 h

Glucanases

 Avicelase III; [Aspergillus aculeatus]

Comp15820_c0

1.1 ± 0.2

5.1 ± 1.2

9.0 ± 0.2

885.0 ± 122.0

 Cell wall glucanase; [Aspergillus niger CBS 513.88]

Comp10145_c0

1.1 ± 0.1

1.3 ± 0.3

1.3 ± 0.2

6.7 ± 6.2

 Endoglucanase-4; [A. niger CBS 513.88] > gi|134,080,248

Comp6428_c0

1.2 ± 0.3

(21.8 ± 22.1)

2.8 ± 0.8

(382.3 ± 473.2)

 1,4-ß-D-glucan cellobiohydrolase A

Comp13421_c0

0 ± 0

1.7 ± 1.0

0 ± 0

70.3 ± 62.9

 1,4-ß-D-glucan cellobiohydrolase B

Comp17477_c0

1.3 ± 0.5

(69.0 ± 81.7)

3.3 ± 0.9

(4173.7 ± 4754.0)

 1,4-ß-D-glucan cellobiohydrolase C; [A. niger CBS 513.88]

Comp19016_c0

1.0 ± 0.1

(146.1 ± 157.3)

25.6 ± 15.8

(64,056.3 ± 87,096)

 ß-glucosidase G [A. niger CBS 513.88]

Comp16375_c2

3.0 ± 0.0

3.66 ± 0.0

1.8 ± 0.2

48.2 ± 29.2

Other polysaccharide depolymerases

 Mannan endo-1,4-β-mannosidase A

Comp7543_c0

1.9 ± 1.2

22.8 ± 8.2

16.0 ± 2.7

1167.2 ± 642.8

 Pectin lyase F; [Aspergillus oryzae RIB40] > gi|391,874,352

Comp10369_c0

11.7 ± 1.3

4.3 ± 1.3

1.6 ± 0.8

(33.1 ± 35.1)

 Endo-α-1,4 polygalactosaminidase; [A. niger CBS513.88]

Comp10792_c0

4.1 ± 0.6

3.0 ± 1.1

1.2 ± 0.3

20.9 ± 9.7

 Rhamnogalacturonate lyase A

Comp15535_c0

4.4 ± 0.5

3.0 ± 1.1

1.2 ± 0.3

20.0 ± 10.9

 Endo-arabinase; [A. niger CBS 513.88]

Comp15955_c0

1.1 ± 0.2

3.4 ± 0.2

3.8 ± 0.2

154.6 ± 153.0

Lipases

 Lipase; [A. niger CBS 513.88] > gi|134,083,043|e

Comp10309_c0

5.8 ± 1.5

6.3 ± 0.8

5.4 ± 1.4

120.2 ± 94.6

 Phospholipase; [A. niger CBS 513.88]

Comp19032_c0

1.5 ± 0.2

1.6 ± 0.4

1.7 ± 0.9

6.0 ± 1.9

Environmental management

 Heat shock trehalose synthase; [A. niger CBS 513.88] > gi|134,084,204|

Comp13560_c0

1.1 ± 0.2

11.1 ± 3.6

75.4 ± 0.7

4333.0 ± 2354.2

 30kD heat shock protein; [A. niger ATCC 1015]

Comp17224_c0

4.9 ± 3.7

3.3 ± 3.3

3.1 ± 1.7

73.3 ± 36.7

 Protease inhibitor; [Talaromyces marneffei ATCC 1822]

Comp16974_c0

54.0 ± 13.9

10.5 ± 4.6

11.4 ± 6.5

165.2± 94.7

 Oxylate decarboxylase OxdC; [Bacillus subtilis (strain 168)]

Comp22183_c0

1.0 ± 0.0

1.8 ± 0.2

5.5 ± 0.2

13.3 ± 3.0

 CDR ABC transporter; [Byssochlamys nivea]

Comp16806_c0

20.6 ± 6.9

9.6 ± 0.6

4.4 ± 1.5

(166.4 ± 175.2)

 ABC multidrug transporter; [A. oryzae RIB40]

Comp15840_c0

1.8 ± 1.1

692.6 ± 203.7

877.9 ± 347.1

915.9 ± 83.8

Regulation

 RNA polymerase II transcription factor [A. niger CBS 513.88] > gi|134,074,447|

Comp7059_c0

1.2 ± 0.3

1.7 ± 0.1

2.7 ± 1.5

(346.1 ± 354.4)

 Fungal specific transcription factor domain protein [A. niger CBS 513.88]

Comp12975_c0

1.8 ± 1.1

(10.6 ± 12.2)

10.0 ± 6.4

3428.3 ± 3025.3

 Transcription factor TFIID complex 145 kDa subunit [Aspergillus kawachii IFO 4308]

Comp14528_c0

1.1 ± 0.1

1.6 ± 0.3

1.4 ± 0.1

49.8 ± 32.6

 C6 transcription factor; [A. niger CBS 513.88]

Comp15729_c0

1.0 ± 0.0

1.3 ± 0.3

2.0 ± 0.2

87.3 ± 74.4

 GATA transcription factor LreB; [Aspergillus fumigatus A1163]

Comp16275_c7

1.0 ± 0.1

2.8 ± 0.0

3.0 ± 1.1

35.1 ± 23.1

 Transcriptional regulator Ngg1; [A. fumigatus Af293] > gi|66,847,840|

Comp15147_c0

1.7 ± 0.5

1.7 ± 1.0

3.6 ± 0.9

(124.0 ± 127.3)

 Sensor histidine kinase; [Aspergillus clavatus NRRL

Comp15130_c0

1.2 ± 0.2

2.3 ± 0.9

2.8 ± 0.0

39.6 ± 11.2

  1. Gene expression levels were calculated from the threshold cycle according to the 2-ΔΔCT method. Values are the mean of two experiments (n = 2), each with three replicates, with standard deviation. a ct and cox5 transcripts, encoding actin and cytochrome c oxidase subunit V, were used as internal references to normalize RNA in each reaction. SD, standard deviation. Values in parentheses were considered too variable to be used in the analysis as the standard deviation was greater than the mean