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Table 2 Relative expression values of genes by Aspergillus aculeatus Asp-4 during growth on PDA

From: Characterization of mechanisms underlying degradation of sclerotia of Sclerotinia sclerotiorum by Aspergillus aculeatus Asp-4 using a combined qRT-PCR and proteomic approach

Putative function

Time after inoculation (Mean +/− SD)

 

12 h

24 h

36 h

48 h

Glucanases

 Avicelase III

8.9 ± 4.0

(64.0 ± 89.0)

566.0 ± 354.3

(124.0 ± 146.3)

 Cell wall glucanase

1.9 ± 0.0

3.0 ± 0.8

6.7 ± 0.8

3.9 ± 0.3

 Endoglucanase-4

1.9 ± 0.3

8.2 ± 5.3

71.0 ± 12.8

33.2 ± 5.6

 1,4-ß-D-glucan cellobiohydrolase A

0.0 ± 0.0

0.0 ± 0.0

0.0 ± 0.0

0.0 ± 0.0

 1,4-ß-D-glucan cellobiohydrolase B

2.6 ± 0.2

30.1 ± 24.9

212.7 ± 159.5

(128.6 ± 149.4)

 1,4-ß-D-glucan cellobiohydrolase C

4.4 ± 1.4

(140.5 ± 140.3)

(2276.8 ± 2527.7)

286.9 ± 148.4

 ß-glucosidase G

1.6 ± 0.8

5.4 ± 2.9

30.2 ± 28.0

14.3 ± 3.8

Other polysaccharide depolymerases

 Mannan endo-1,4-β-mannosidase A

48.9 ± 19.1

42.0 ± 1.8

145.2 ± 98.7

(36.1 ± 36.1)

 Pectin lyase F

6.1 ± 4.1

4.3 ± 2.2

(15.6 ± 17.5)

2.1 ± 0.1

 Endo-α-1,4 polygalactosaminidase

1.5 ± 0.5

3.3 ± 1.0

6.7 ± 2.7

2.9 ± 0.0

 Rhamnogalacturonate lyase A

1.5 ± 0.5

3.5 ± 1.1

5.7 ± 2.0

2.9 ± 0.2

 Endo-arabinase

2.0 ± 0.4

6.1 ± 5.5

99.8 ± 87.5

25.9 ± 3.2

Lipases

 Lipase

1.8 ± 1.2

13.9 ± 4.9

65.4 ± 61.8

17.8 ± 1.8

 Phospholipase

1.0 ± 0.0

2.1 ± 1.3

4.9 ± 1.4

5.2 ± 1.0

Environmental management

 Heat shock trehalose synthase

1.7 ± 0.1

50.0 ± 40.9

(931.1 ± 1030.7)

425.8 ± 78.0

 30kD heat shock protein

9.3 ± 1.7

17.1 ± 3.9

92.9 ± 1.7

256.8 ± 21.6

 Protease inhibitor

10.5 ± 1.7

3.2 ± 0.6

9.2 ± 1.7

1.3 ± 0.4

 Oxylate decarboxylase OxdC

2.5 ± 0.1

13.2 ± 0.0

26.0 ± 10.1

12.9 ± 2.8

 CDR ABC transporter

1.1 ± 0.2

7.1 ± 6.7

(76.1 ± 80.3)

14.4 ± 0.4

 ABC multidrug transporter

2.0 ± 0.5

371.8 ± 211.1

1487.8 ± 470.7

9339.8 ± 1873.5

Regulation

 RNA polymerase II transcription factor

4.0 ± 0.8

26.1 ± 8.4

190.8 ± 171.8

61.3 ± 7.6

 Fungal specific transcription factor domain protein

1.7 ± 0.2

78.3 ± 47.2

(383.7 ± 449.8)

486.6 ± 282.4

 Transcription factor TFIID complex 145 kDa subunit

1.8 ± 0.5

6.9 ± 3.6

15.1 ± 11.5

27.8 ± 6.3

 C6 transcription factor

2.2 ± 0.4

14.2 ± 5.0

52.6 ± 44.0

21.8 ± 0.6

 GATA transcription factor LreB

3.4 ± 1.1

7.0 ± 5.1

54.5 ± 20.0

43.2 0.3

 Transcriptional regulator Ngg1

2.8 ± 1.0

55.4 ± 33.2

199.8 ± 104.4

172.9 ± 3.0

 Sensor histidine kinase

6.4 ± 0.2

(6.3 ± 6.2)

68.3 ± 50.9

22.5 ± 3.0

  1. Gene expression levels were calculated from the threshold cycle according to the 2-ΔΔCT method. Values are the mean of two experiments (n = 2), each with three replicates, with standard deviation. a ct and cox5 transcripts, encoding actin and cytochrome c oxidase subunit V, were used as internal references to normalize RNA in each reaction. SD, standard deviation. Values in parentheses were considered too variable to be used in the analysis as the standard deviation was greater than the mean. See Table 1 for the gene ID