Table 1 Knockout strains derived by pessimistic and optimistic models on the core E.coli metabolic network
From: Robust mutant strain design by pessimistic optimization
K | Knockouts | Succinate production | |
|---|---|---|---|
P-ROOM | 3 | 6pg →ru5p + co2 + nadph, oac + accoa →cit, ac →ac(ext) | 76.97 |
4 | g6p →6pg + nadph, oac + accoa →cit, suc ⇔fum + fadh2, ac →ac(ext) | 82.36 | |
5 | g6p →6pg + nadph, mal →pyr + co2 + nadph, 3pg + glu →ser + akg + nadh, fadh2 + 0.5o2 →2atp, nadh ⇔nadph | 107.07 | |
P-OptKnock | 3 | 6pg →ru5p + co2 + nadph, oac + accoa → cit, ac →ac(ext) | 76.97 |
4 | g6p →6pg + nadph, oac + accoa →cit, suc ⇔fum + fadh2, ac →ac(ext) | 82.36 | |
5 | g6p →6pg + nadph, mal →pyr + co2 + nadph, 3pg + glu →ser + akg + nadh, fadh2 + 0.5o2 →2atp, nadh ⇔nadph | 107.07 | |
ROOM | 3 | dhap ⇔gap, g6p →6pg + nadph, fadh2 + 0.5o2 →2atp | 93.92 |
4 | dhap ⇔gap, g6p →6pg + nadph, fadh2 + 0.5o2 →2atp, glyc(ext) → | 108.22 | |
5 | g6p →6pg + nadph, mal →pyr + co2 + nadph, 3pg + glu →ser + akg + nadh, fadh2 + 0.5o2 →2atp, nadh ⇔nadph | 115.58 | |
OptKnock | 3 | g6p →6pg + nadph, mal →pyr + co2 + nadph, nadh ⇔nadph | 110.179 |
4 | g6p →6pg + nadph, mal →pyr + co2 + nadph, 3pg + glu →ser + akg + nadh, nadh ⇔ nadph | 123.314 | |
5 | g6p →6pg + nadph, 3pg + glu →ser + akg + nadh, nadh ⇔nadph, glyc ⇔glyc(ext), ac(ext) → | 129.786 |