Comparative mitochondrial proteomic, physiological, biochemical and ultrastructural profiling reveal factors underpinning salt tolerance in tetraploid black locust (Robinia pseudoacacia L.)

Luo, Qiuxiang; Peng, Mu; Zhang, Xiuli; Lei, Pei; Ji, Ximei; Chow, Wahsoon; Meng, Fanjuan; Sun, Guanyu

doi:10.1186/s12864-017-4038-2

Table 2 Differentially expressed proteins spots in mitochondria of mesophyll cells of diploid (2×) and tetraploid (4×) R. pseudoacacia using 2D–Gel Analysis under 0, 250 mM and 500 mM NaCl treatment

From: Comparative mitochondrial proteomic, physiological, biochemical and ultrastructural profiling reveal factors underpinning salt tolerance in tetraploid black locust (Robinia pseudoacacia L.)

Spot no.^a	Protein Name	Species	Accession^b	Theor pI/KDa^c	Exper pI/KDa^d	Score^e	SC (%)^f	PN^g
Oxidative phosphorylation (OXPHOS) system
34	NADH-ubiquinone oxidoreductase (complex I) 75kDa subunit	Zea mays	gi\|195648210	6.1/89.0	4.78/66	79	3%	2	T
178	NADH dehydrogenase (complex I) iron-sulfur protein 1	Vitis vinifera	gi\|225453076	6.5/81.8	6.1/71	193	19%	14	D
190	putative NADH dehydrogenase (complex I)	Bonyunia minor	gi\|6686959	6.0/5.18	6.53/27	580	18%	8	T
230	NAD⁺ binding site	Glycine max	gi\|356525205	5.6/53.8	5.9/45	199	34%	26	D
291	family protein (complex I)	Arabidopsis lyrata L. subsp	gi\|297312256	9.88/10.1	5.47/25	45	7%	9	D
215	Cytochrome c reductase (complex III) Mitochondrial processing peptidase subunit β	Glycine max	gi\|356558971	6.27/58	6.2/50	399	29%	27	D
53	ATP synthase (complex V) α subnunit	Arabidopsis thaliana	gi\|15219234	5.11/69	5.52/60	203	12%	5	T
71	ATP synthase (complex V) β subnunit	Kalidium foliatum	gi\|118429132	4.93/54.1	5.02/52	264	13%	5	T
88	ATP synthase (complex V) β subnunit	Stirlingia latifolia	gi\|3850914	5.09/52.8	5.51/45	548	31%	9	T
207	ATP synthase (complex V) α subnunit	Manihot esculenta	gi\|169794058	5.13/55	5.1/52	733	35%	24	A
209	ATP synthase (complex V) α subnunit	Vigna radiata	gi\|289066833	5.1/55	5.1/53	382	26%	24	D
211	ATP synthase (complex V) βsubnunit	Glycine max	gi\|356575458	4.9/54	4.9/52	626	55%	33	D
213	ATP synthase (complex V) α subnunit	Manihot esculenta	gi\|169794058	5.22/55	5.26/52	425	27%	20	A
220	ATP synthase (complex V) βsubnunit	Glycine max	gi\|356536246	5.8/59	5.3/48	152	36%	21	D
223	ATP synthase (complex V) βsubnunit	Glycine max	gi\|91214126	5.29/53	5.37/47	903	53%	31	D
198	Cell division protein ftsH	Ricinus communis	gi\|255558698	6.43/75	5.1/64	258	35%	27	D
216	ATP₁ gene product	Lotus japonicus	gi\|372450305	6.01/55	6.44/50	509	45%	27	D
285	Chromosome segregation ATPases (Myosin-like protein)	Medicago truncatula	gi\|357463145	4.65/206	5.5/27	52	14%	25	A
Transcription, translation; DNA-binding proteins
33	pentatricopeptide repeat-containing protein	Setaria italica	gi\|514771455	8.17/59.2	5.78/64	33	2%	1	T
171	Ankyrin repeat domain-containing protein	Medicago truncatula	gi\|357500765	6.80/53	5.22/24	53	33%	12	D
175	Elongation factor G	Vitis viniferad	gi\|359496425	5.52/85	5.2/86	328	41%	34	D
253	ATP-dependent RNA helicase	Brachypodium distachyon	gi\|357126966	9.24/49	6.28/37	58	36%	12	A
181	zinc ion binding protein	Arabidopsis thaliana	gi\|18399026	9.12/31	5.9/72	55	41%	10	D
298	Calcium-binding EF-hand family protein	Theobroma cacao	gi\|508777222	4.82/16.5	6.14/23	31	4%	1	D
299	Calcium-binding protein	Solanum lycopersicum	gi\|460378369	4.90/16.5	5.14/23	31	4%	1	D
232	kinesin heavy chain	Ricinus communis	gi\|255537481	7.88/12	6.0/41	55	22%	18	A
315	50S ribosomal protein L21, mitochondrial	Arabidopsis thaliana	gi\|29839556	5.52/30.9	6.4/17	43	30%		D
350	ribosomal protein S8	Monomastix	gi\|224179526	10.1/16.4	6.46/14	29	8%	2	D
317	DNA binding protein, putative	Ricinus communis	gi\|223531593	5.77/35.1	4.96/16	44	15%		D
343	pentatricopeptide repeat-containing protein	Setaria italica	gi\|514771455	8.17/59.1	5.67/26	35	2%	1	D
Chaperones and protein processing
282	Heat shock protein	Medicago truncatula	gi\|357503161	5.10/71	6.75/27	118	22%	17	A
149	Heat shock protein	Zea mays	gi\|162461165	7.88/26	6.3/26	57	35%	12	D
201	protein disulfide-isomerase	Glycine max	gi\|356554621	5.28/56	5.0/62	47	9%	7	D
231	A1 cistron-splicing factor AAR2	Arabidopsis thaliana	gi\|30697362	5.05/41	5.89/41	54	39%	12	D
Transport
39	mitochondrial substrate carrier	Volvox carteri f. nagariensis	gi\|300265179	6.25/36	6.06/61	43	11%	1	T
154	adenosine deaminase-like	Glycine max	gi\|356521975	5.80/25	5.28/25	46	17%	8	D
339	eukaryotic translation initiation factor 2c	Ricinus communis	gi\|223543695	9.29/110	6.04/14	47	3%	7	T
Pyruvate decarboxylation and citric acid cycle
265	malate dehydrogenase	Glycine max	gi\|373432589	5.91/35	6.45/33	147	40%	15	A
Metabolism
86	Alanine aminotransferase	Medicago truncatula	gi\|357485703	5.78/54.0	4.45/46	120	6%	2	T
113	Glutamine synthetase	Ricinus communis	gi\|255551511	6.69/48.1	5.30/39	49	8%	1	T
119	Glutamine synthetase	Canavalia lineata	gi\|6578120	6.28/47.6	5.46/39	112	9%	3	T
121	putative alcohol dehydrogenase	Betula pendula	gi\|6723484	8.58/20.1	6.51/38	76	5%	1	T
142	P-loop containing nucleoside triphosphate hydrolases superfamily protein	Arabidopsis thaliana	gi\|15219376	6.81/48.3	6.21/34	191	6%	2	T
346	fucosyltransferase CAZy family GT37-like protein	Selaginella moellendorffii	gi\|300143173	7.91/58	5.97/16	40	20%	1	D
Defense, stress, detoxification
111	1,3-β-glucan synthase subunit	Fragaria vesca	gi\|470139357	8.99/204	6.57/39	41	4%	7	T
186	BZIP transcription factor	Medicago truncatula	gi\|357486627	8.65/24	6.09/71	50	36%	11	D
329	disease resistance RPP8-like protein 3-like	Glycine max	gi\|356538242	8.76/104	6.82/14	24	3%	1	D
203	L-ascorbate peroxidase	Cicer arietinum	gi\|502145236	5.65/27.1	5.04/26	141	12%	2	T
217	lectin	Astragalus falcatus	gi\|3819113	3.85/9.80	5.92/24	66	8%	1	T
Unknown function
163	predicted protein	Glycine max	gi\|168064083	9.36/27	5.23/24	49	48%	9	D
281	predicted protein	Physcomitrella patens	gi\|168070396	6.56/47	4.9/28	54	31%	14	A
221	predicted protein	Populus trichocarpa	gi\|224086409	10.8/14.4	5.26/24	42	8%	1	T
254	predicted protein	Populus trichocarpa	gi\|224109480	5.31/47.2	6.11/37	65	17%	8	D
30	uncharacterized protein	Zea mays	gi\|308082002	11.06/24.8	5.98/65	50	39%	8	T
349	uncharacterized protein	Glycine max	gi\|359807168	6.24/32.7	6.24/24	384	22%	6	D
112	hypothetical protein	Prunus persica	gi\|462400783	6.84/52	5.11/39	351	16%	6	T
183	hypothetical protein	Musa acuminata var. zebrina	gi\|1491726	7.37/37.2	6.58/28	114	11%	3	T
348	hypothetical protein	Vitis vinifera	gi\|147817756	5.54/33.8	6.23/22	148	24%	4	D
129	unknown	Medicago truncatula	gi\|217073023	7.67/36.5	5.05/37	286	13%	4	T
228	unknown	Lotus japonicus	gi\|388515001	5.74/21.5	5.48/22	148	12%	2	T
274	unknown	Medicago truncatula	gi\|217071716	4.66/18.0	5.20/29	337	49%	5	D
Miscellancous Proteins
246	Ribulose 1,5-bisphosphate carboxylase	Loeseneriella	gi\|9909955	6.04/52.3	5.39/19	270	9%	4	T
258	Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit	Ulmus alata	gi\|523330	6.05/53.1	5.86/16	315	12%	5	D
264	Ribulose bisphosphate carboxylase large chain	Prostanthera nivea	gi\|671611	6.33/52.8	5.01/15	337	8%	5	T
307	Ribulose bisphosphate carboxylase large chain	Medicago truncatula	gi\|357502811	6.13/53	6.33/20	235	21%	14	A
312	Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit	Lotus japonicus	gi\|13518420	6.22/51	5.79/19	249	24%	15	A

^a Assigned spot number as indicated in Figs. 6 and 7. ^b Accession numbers according to the NCBIInr database. ^c Theoretical pI and masses (kDa) and of identified proteins. ^d Experimental pI and masses (kDa) and of identified proteins. ^e Mascot protein score reported after searching against the NCBInr database. ^f Sequence coverage. ^g Number of peptides sequenced. ^h Mean of relative protein abundance and standard error. D, Protein spots only detected in diploid black locust. T, Protein spots only detected in tetraploid black locust. A, Protein spots detected in diploid and tetraploid black locust. Six treatments including 1, 5, and 10 days after 500 mM NaCl treatment were performed

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