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Table 2 Differentially expressed proteins spots in mitochondria of mesophyll cells of diploid (2×) and tetraploid (4×) R. pseudoacacia using 2D–Gel Analysis under 0, 250 mM and 500 mM NaCl treatment

From: Comparative mitochondrial proteomic, physiological, biochemical and ultrastructural profiling reveal factors underpinning salt tolerance in tetraploid black locust (Robinia pseudoacacia L.)

Spot no.a Protein Name Species Accessionb Theor pI/KDac Exper pI/KDad Scoree SC
(%)f
PNg Relative V% ±Seh  
Oxidative phosphorylation (OXPHOS) system
 34 NADH-ubiquinone oxidoreductase
(complex I) 75kDa subunit
Zea mays gi|195648210 6.1/89.0 4.78/66 79 3% 2 T
 178 NADH dehydrogenase (complex I)
iron-sulfur protein 1
Vitis vinifera gi|225453076 6.5/81.8 6.1/71 193 19% 14 D
 190 putative NADH dehydrogenase (complex I) Bonyunia minor gi|6686959 6.0/5.18 6.53/27 580 18% 8 T
 230 NAD+ binding site Glycine max gi|356525205 5.6/53.8 5.9/45 199 34% 26 D
 291 family protein (complex I) Arabidopsis lyrata L. subsp gi|297312256 9.88/10.1 5.47/25 45 7% 9 D
 215 Cytochrome c reductase (complex III)
Mitochondrial processing peptidase
subunit β
Glycine max gi|356558971 6.27/58 6.2/50 399 29% 27 D
 53 ATP synthase (complex V)
α subnunit
Arabidopsis thaliana gi|15219234 5.11/69 5.52/60 203 12% 5 T
 71 ATP synthase (complex V)
β subnunit
Kalidium foliatum gi|118429132 4.93/54.1 5.02/52 264 13% 5 T
 88 ATP synthase (complex V)
β subnunit
Stirlingia latifolia gi|3850914 5.09/52.8 5.51/45 548 31% 9 T
 207 ATP synthase (complex V)
α subnunit
Manihot esculenta gi|169794058 5.13/55 5.1/52 733 35% 24 A
 209 ATP synthase (complex V)
α subnunit
Vigna radiata gi|289066833 5.1/55 5.1/53 382 26% 24 D
 211 ATP synthase (complex V)
βsubnunit
Glycine max gi|356575458 4.9/54 4.9/52 626 55% 33 D
 213 ATP synthase (complex V)
α subnunit
Manihot esculenta gi|169794058 5.22/55 5.26/52 425 27% 20 A
 220 ATP synthase (complex V)
βsubnunit
Glycine max gi|356536246 5.8/59 5.3/48 152 36% 21 D
 223 ATP synthase (complex V)
βsubnunit
Glycine max gi|91214126 5.29/53 5.37/47 903 53% 31 D
 198 Cell division protein ftsH Ricinus communis gi|255558698 6.43/75 5.1/64 258 35% 27 D
 216 ATP1 gene product Lotus japonicus gi|372450305 6.01/55 6.44/50 509 45% 27 D
 285 Chromosome segregation ATPases
(Myosin-like protein)
Medicago truncatula gi|357463145 4.65/206 5.5/27 52 14% 25 A
Transcription, translation; DNA-binding proteins
 33 pentatricopeptide repeat-containing protein Setaria italica gi|514771455 8.17/59.2 5.78/64 33 2% 1 T
 171 Ankyrin repeat domain-containing protein Medicago truncatula gi|357500765 6.80/53 5.22/24 53 33% 12 D
 175 Elongation factor G Vitis viniferad gi|359496425 5.52/85 5.2/86 328 41% 34 D
 253 ATP-dependent RNA helicase Brachypodium distachyon gi|357126966 9.24/49 6.28/37 58 36% 12 A
 181 zinc ion binding protein Arabidopsis thaliana gi|18399026 9.12/31 5.9/72 55 41% 10 D
 298 Calcium-binding EF-hand family protein Theobroma cacao gi|508777222 4.82/16.5 6.14/23 31 4% 1 D
 299 Calcium-binding protein Solanum lycopersicum gi|460378369 4.90/16.5 5.14/23 31 4% 1 D
 232 kinesin heavy chain Ricinus communis gi|255537481 7.88/12 6.0/41 55 22% 18 A
 315 50S ribosomal protein L21, mitochondrial Arabidopsis thaliana gi|29839556 5.52/30.9 6.4/17 43 30%   D
 350 ribosomal protein S8 Monomastix gi|224179526 10.1/16.4 6.46/14 29 8% 2 D
 317 DNA binding protein, putative Ricinus communis gi|223531593 5.77/35.1 4.96/16 44 15%   D
 343 pentatricopeptide repeat-containing protein Setaria italica gi|514771455 8.17/59.1 5.67/26 35 2% 1 D
Chaperones and protein processing
 282 Heat shock protein Medicago truncatula gi|357503161 5.10/71 6.75/27 118 22% 17 A
 149 Heat shock protein Zea mays gi|162461165 7.88/26 6.3/26 57 35% 12 D
 201 protein disulfide-isomerase Glycine max gi|356554621 5.28/56 5.0/62 47 9% 7 D
 231 A1 cistron-splicing factor AAR2 Arabidopsis thaliana gi|30697362 5.05/41 5.89/41 54 39% 12 D
Transport
 39 mitochondrial substrate carrier Volvox carteri f. nagariensis gi|300265179 6.25/36 6.06/61 43 11% 1 T
 154 adenosine deaminase-like Glycine max gi|356521975 5.80/25 5.28/25 46 17% 8 D
 339 eukaryotic translation initiation factor 2c Ricinus communis gi|223543695 9.29/110 6.04/14 47 3% 7 T
Pyruvate decarboxylation and citric acid cycle
 265 malate dehydrogenase Glycine max gi|373432589 5.91/35 6.45/33 147 40% 15 A
Metabolism
 86 Alanine aminotransferase Medicago truncatula gi|357485703 5.78/54.0 4.45/46 120 6% 2 T
 113 Glutamine synthetase Ricinus communis gi|255551511 6.69/48.1 5.30/39 49 8% 1 T
 119 Glutamine synthetase Canavalia lineata gi|6578120 6.28/47.6 5.46/39 112 9% 3 T
 121 putative alcohol dehydrogenase Betula pendula gi|6723484 8.58/20.1 6.51/38 76 5% 1 T
 142 P-loop containing nucleoside triphosphate hydrolases superfamily protein Arabidopsis thaliana gi|15219376 6.81/48.3 6.21/34 191 6% 2 T
 346 fucosyltransferase CAZy family GT37-like protein Selaginella moellendorffii gi|300143173 7.91/58 5.97/16 40 20% 1 D
Defense, stress, detoxification
 111 1,3-β-glucan synthase subunit Fragaria vesca gi|470139357 8.99/204 6.57/39 41 4% 7 T
 186 BZIP transcription factor Medicago truncatula gi|357486627 8.65/24 6.09/71 50 36% 11 D
 329 disease resistance RPP8-like protein 3-like Glycine max gi|356538242 8.76/104 6.82/14 24 3% 1 D
 203 L-ascorbate peroxidase Cicer arietinum gi|502145236 5.65/27.1 5.04/26 141 12% 2 T
 217 lectin Astragalus falcatus gi|3819113 3.85/9.80 5.92/24 66 8% 1 T
Unknown function
 163 predicted protein Glycine max gi|168064083 9.36/27 5.23/24 49 48% 9 D
 281 predicted protein Physcomitrella patens gi|168070396 6.56/47 4.9/28 54 31% 14 A
 221 predicted protein Populus trichocarpa gi|224086409 10.8/14.4 5.26/24 42 8% 1 T
 254 predicted protein Populus trichocarpa gi|224109480 5.31/47.2 6.11/37 65 17% 8 D
 30 uncharacterized protein Zea mays gi|308082002 11.06/24.8 5.98/65 50 39% 8 T
 349 uncharacterized protein Glycine max gi|359807168 6.24/32.7 6.24/24 384 22% 6 D
 112 hypothetical protein Prunus persica gi|462400783 6.84/52 5.11/39 351 16% 6 T
 183 hypothetical protein Musa acuminata var. zebrina gi|1491726 7.37/37.2 6.58/28 114 11% 3 T
 348 hypothetical protein Vitis vinifera gi|147817756 5.54/33.8 6.23/22 148 24% 4 D
 129 unknown Medicago truncatula gi|217073023 7.67/36.5 5.05/37 286 13% 4 T
 228 unknown Lotus japonicus gi|388515001 5.74/21.5 5.48/22 148 12% 2 T
 274 unknown Medicago truncatula gi|217071716 4.66/18.0 5.20/29 337 49% 5 D
Miscellancous Proteins
 246 Ribulose 1,5-bisphosphate carboxylase Loeseneriella gi|9909955 6.04/52.3 5.39/19 270 9% 4 T
 258 Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit Ulmus alata gi|523330 6.05/53.1 5.86/16 315 12% 5 D
 264 Ribulose bisphosphate carboxylase large chain Prostanthera nivea gi|671611 6.33/52.8 5.01/15 337 8% 5 T
 307 Ribulose bisphosphate carboxylase large chain Medicago truncatula gi|357502811 6.13/53 6.33/20 235 21% 14 A
 312 Ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit Lotus japonicus gi|13518420 6.22/51 5.79/19 249 24% 15 A
  1. a Assigned spot number as indicated in Figs. 6 and 7. b Accession numbers according to the NCBIInr database. c Theoretical pI and masses (kDa) and of identified proteins. d Experimental pI and masses (kDa) and of identified proteins. e Mascot protein score reported after searching against the NCBInr database. f Sequence coverage. g Number of peptides sequenced. h Mean of relative protein abundance and standard error. D, Protein spots only detected in diploid black locust. T, Protein spots only detected in tetraploid black locust. A, Protein spots detected in diploid and tetraploid black locust. Six treatments including 1, 5, and 10 days after 500 mM NaCl treatment were performed