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Table 1 Microsporidia (MS) Facilispora margolisi infections in adult female salmon lice Lepeophtheirus salmonis (F0) and their F1 larval progeny as determined by PCR

From: Effects of the vertically transmitted microsporidian Facilispora margolisi and the parasiticide emamectin benzoate on salmon lice (Lepeophtheirus salmonis)

EXP Source of F0 lice F0 individual (MS+ / total) F1 samples (MS+ / total)
Cephalothorax Egg String F0 positive F0 negative
 1 Chum Salmon 12/20 12/20 11/12 (pools) 0/8
 2 Chum Salmon 14/24 13/24 28/39 0/42
 3 Atlantic Salmon 25/40 N/A N/A N/A
 4 Atlantic Salmon 23/40 22/40 2/2 (pools) 0/2
Total   74/124 (60%) 47/84 (56%) 28/39 (72%)a 0/42 (0%)a
  1. In experiment (Exp.) 1, each F1 nauplius and copepodid pool originated from a single F0 egg string. In Exp. 2, F1 chalimus II staged lice had developed from pools of MS+ or MS- eggs derived from F0 females. In Exp. 3, F1 nauplius and copepodid pools were reserved for in situ hybridizations. In Exp. 4, 40 larval cultures derived from MS+ or MS- F0 females were divided into 16 pools for each of MS+ and MS- groups, 14 for microarray analysis and 2 for PCR confirmation of F. margolisi (PCR confirmation pools shown in the table). All PCR-positive results in the egg string were also positive in the cephalothorax. aTotals only include individuals, not pools