EXP
|
Source of F0 lice
|
F0 individual (MS+ / total)
|
F1 samples (MS+ / total)
|
---|
Cephalothorax
|
Egg String
|
F0 positive
|
F0 negative
|
---|
1
|
Chum Salmon
|
12/20
|
12/20
|
11/12 (pools)
|
0/8
|
2
|
Chum Salmon
|
14/24
|
13/24
|
28/39
|
0/42
|
3
|
Atlantic Salmon
|
25/40
|
N/A
|
N/A
|
N/A
|
4
|
Atlantic Salmon
|
23/40
|
22/40
|
2/2 (pools)
|
0/2
|
Total
| |
74/124 (60%)
|
47/84 (56%)
|
28/39 (72%)a
|
0/42 (0%)a
|
- In experiment (Exp.) 1, each F1 nauplius and copepodid pool originated from a single F0 egg string. In Exp. 2, F1 chalimus II staged lice had developed from pools of MS+ or MS- eggs derived from F0 females. In Exp. 3, F1 nauplius and copepodid pools were reserved for in situ hybridizations. In Exp. 4, 40 larval cultures derived from MS+ or MS- F0 females were divided into 16 pools for each of MS+ and MS- groups, 14 for microarray analysis and 2 for PCR confirmation of F. margolisi (PCR confirmation pools shown in the table). All PCR-positive results in the egg string were also positive in the cephalothorax. aTotals only include individuals, not pools