EXP | Source of F0 lice | F0 individual (MS+ / total) | F1 samples (MS+ / total) |
---|
Cephalothorax | Egg String | F0 positive | F0 negative |
---|
 1 | Chum Salmon | 12/20 | 12/20 | 11/12 (pools) | 0/8 |
 2 | Chum Salmon | 14/24 | 13/24 | 28/39 | 0/42 |
 3 | Atlantic Salmon | 25/40 | N/A | N/A | N/A |
 4 | Atlantic Salmon | 23/40 | 22/40 | 2/2 (pools) | 0/2 |
Total | Â | 74/124 (60%) | 47/84 (56%) | 28/39 (72%)a
| 0/42 (0%)a
|
- In experiment (Exp.) 1, each F1 nauplius and copepodid pool originated from a single F0 egg string. In Exp. 2, F1 chalimus II staged lice had developed from pools of MS+ or MS- eggs derived from F0 females. In Exp. 3, F1 nauplius and copepodid pools were reserved for in situ hybridizations. In Exp. 4, 40 larval cultures derived from MS+ or MS- F0 females were divided into 16 pools for each of MS+ and MS- groups, 14 for microarray analysis and 2 for PCR confirmation of F. margolisi (PCR confirmation pools shown in the table). All PCR-positive results in the egg string were also positive in the cephalothorax. aTotals only include individuals, not pools