Effects of the vertically transmitted microsporidian Facilispora margolisi and the parasiticide emamectin benzoate on salmon lice (Lepeophtheirus salmonis)

Table 2 Unique SwissProt IDs for transcripts differentially expressed by EMB exposure and F. margolisi infection

Functional annotation term	Accession ID	Count (# Unique SwissProt IDs)			P-value of enrichment
Functional annotation term	Accession ID	MS	EMB	Shared	MS	EMB
Overexpressed contigs
ATP binding	GO:0005524	25	18	10	4E-04	2E-05
Chaperone	Keywords	16	10	10	2E-08	3E-06
Chaperonin-containing T-complex	GO:0005832	4	4	4	3E-04	5E-05
Chaperonin Cpn60/TCP-1	IPR002423	9	6	6	3E-11	2E-07
Cytosol	GO:0005829	15	11	7	0.002	0.001
Nucleotide-binding	Keywords	31	17	9	2E-05	8E-04
Protein folding	GO:0006457	17	11	11	6E-10	3E-07
Protein metabolic process	GO:0019538	36	26	15	4E-04	1E-05
Underexpressed contigs
Disulfide bond	Keywords	20	12	9	2E-05	8E-05
Extracellular region	GO:0005576	17	8	7	1E-05	0.003
Glycoprotein	Keywords	21	15	13	0.005	1E-04
Integral to membrane	GO:0016021	32	17	14	0.005	0.005
Peptidase activity, acting on L-amino acid peptides	GO:0070011	13	8	7	7E-04	0.002
Peptidase S1 and S6, chymotrypsin/Hap	IPR001254	6	4	3	6E-04	0.004
Protease	Keywords	13	8	7	8E-04	0.002
Serine protease	Keywords	8	6	5	8E-05	9E-05
Secreted	Keywords	13	8	7	1E-04	5E-04
Substrate-specific transmembrane transporter activity	GO:0022891	10	6	5	0.02	0.03
Transmembrane	Keywords	32	17	14	3E-04	0.001

Transcripts (unique SwissProt IDs only) from each main effect list (2-way ANOVA; p < 0.01; FC ≥ 1.5 main effect and no interaction effect) were analyzed independently and then compared for similar functional enrichments including Gene Ontology (GO), InterPro (IPR), and SP_PIR_Keyword (Keywords; [61,62,63]. The numbers of transcripts responding to both stressors are displayed in the shared count column. See Additional files 1 and 2 for transcript IDs and SwissProt accessions for each functional term

ISSN: 1471-2164