Fig. 4

Gene organization of the dksA and XCV1265 regions. a and c dksA and XCV1265 loci of Xe. All six reading frames are shown. Grey: annotated CDS; orange dashes: peptide-data detected by MS/MS; green hexagons: possible translation start codons of dksA and XCV1265. b and d Analysis of potential translation start codons of DksA and XCV1265. Total protein extracts of Xe 85–10 containing pBRM-P(dksA), pBRM-P(dksA_GTG1), pBRM-P (dksA_GTG2), pBRM-P (dksA_GTG3), pBRM-P (XCV1265), pBRM-P (XCV1265_ATG1), pBRM-P (XCV1265_ATG2) or an empty vector (−) were separated by 12% SDS PAGE and analyzed by immunoblotting using a c-Myc-specific antibody. As loading control, membranes were reacted with a GroEL-specific antibody. Experiments were repeated at least twice with similar results