Fig. 6
Deletion of conserved T6SS components has no effect on Xe virulence. a Schematic representation of the two genomic T6SS loci in Xe. Gene numbers and commonly used gene names of T6SS components identified in Xe 85–10 are given. Dashed lines mark genes deleted in this study. b Xe strains 85–10, 85-10ΔTssFGH1ΔTssFGH2 and 85-10ΔTssI1ΔTssI2 were inoculated into susceptible pepper plants (ECW), resistant pepper plants (ECW-10R) and susceptible tomato plants with an OD600 of 0.1. Phenotypes were documented 7 days post inoculation (7 dpi, ECW), 2 dpi (ECW-10R) and 8 dpi (tomato). c Xe strains 85–10, 85-10ΔTssI1ΔTssI2 and 85-10ΔhrcN (T3SS mutant) were inoculated in ECW plants with an OD600 of 4 × 10−5. Bacterial multiplication in leaves was monitored for 12 days. d Xe strains 85–10, 85-10ΔTssFGH1ΔTssFGH2, 85-10ΔavrBs2 and 85-10ΔTssFGH1ΔTssFGH2ΔavrBs2 were inoculated with an OD600 of 0.1 into leaves of pepper plants (ECW, ECW-10R, ECW-20R) and tomato plants. Phenotypes were documented 6 dpi (ECW), 2 dpi (ECW-10R, ECW-20R) and 9 dpi (tomato). Leaves of ECW-10R and ECW-20R plants were bleached in EtOH for better visualization of cell death reactions. Experiments were repeated twice with similar results