Fig. 4

Relative expression of selected Phlomis purpurea transcripts by quantitative real-time PCR at three post-challenge times. The following selected genes are putatively related to P. purpurea response to the pathogen Gene1: wall-associated receptor-kinase 5-like; gene2: cinnamyl-alcohol dehydrogenase; gene3: hydroxyproline-rich glycoprotein; gene4: cyanidin-3-O-glucoside 2-O-glucoronosyltransferase-like; gene5: resistance protein rgc2; gene6: subtilisin-like protease; gene7: calcium-binding mitochondrial carrier protein aralar1-like; gene8: serine-threonine protein plant. Transcript quantitative expression was normalized with the reference genes β-actin, succinate dehydrogenase and elongation factor1α. Error bars correspond to the standard deviation of the mean of four technical replicates