A molecular view of the normal human thyroid structure and function reconstructed from its reference transcriptome map

Table 6 Selected genes to validate the thyroid transcriptome map in vitro through Real-Time RT-PCR

Gene symbol	Gene name	EEV	SD	ER	Ct mean	OR
TG	Thyroglobulin	8,662.34	33.14	11.51	17.55	7.41
RPL41	Ribosomal protein L41	5,903.29	51.15	7.85	17.11	10.056
B2M	Beta-2-microglobulin	2,988.28	64.22	3.97	18.22	4.66
ACTB	Actin, beta	2,338.95	63.50	3.11	19.38	2.085
TPO	Thyroid peroxidase	2,245.11	54.57	2.98	19.38	2.08
IYD	Iodotyrosine deiodinase	890.07	65.51	1.18	21.16	0.61
*SDC2*	Syndecan 2	752.49	59.44	1	20.44	1
TSHR	Thyroid stimulating hormone receptor	487.64	91.14	0.65	21.34	0.54
SERPINF1	Serpin family F member 1	320.48	65.03	0.43	23.63	0.11
MYH9	Myosin, heavy chain 9, non-muscle	315.95	104.13	0.42	24.19	0.074
BACE2	Beta-site APP-cleaving enzyme 2	105.67	47.06	0.14	24.75	0.050
REEP3	Receptor accessory protein 3	85.88	108.64	0.11	N/A	N/A
YKT6	YKT6 v-SNARE homolog (S. cerevisiae)	29.63	96.23	0.04	26.08	0.020
NTNG1	Netrin G1	17.89	83.44	0.024	32.51	2.33 × 10⁻⁴
RIPPLY3	Ripply transcriptional repressor 3	16.88	97.44	0.022	33.10	1.55 × 10⁻⁴
NPTX1	Neuronal pentraxin 1	16.77	79.37	0.022	33.37	1.28 × 10⁻⁴
TBX18	T-box 18	11.52	100.73	0.015	28.63	0.0034

In bold the gene chosen as reference. From left to right: official gene symbol of the selected gene; official full gene name; expected expression value (EEV), i.e. mean linear expression value for the gene as provided by TRAM software; standard deviation (SD) as percentage of the mean; expected ratio (ER) between target and reference gene expression value; threshold cycle (Ct) mean provided by BioRad CFX Manager Software 2.1 manually positioning the threshold line; observed ratio (OR) determined between each target gene and the reference gene using the 2^∆Ct method, according to the formula: 2^ΔCt = 2 ^{Ct ref. - Ct target}

ISSN: 1471-2164