Fig. 3

Experimental validation of AS events in Phaseolus vulgaris and Glycine max genes. Each selected gene, with conserved AS events in both species, is shown in a different panel: a) Phvul.011G190600, Glyma.13G187200; b) Phvul.008G270400, Glyma.02G293300; c) Phvul.007G262600, Glyma.09G104200; d) Phvul.005G127700, Glyma.12G181300; e) Phvul.003G077300, Glyma.07G259200; f) Phvul.002G298304, Glyma.08G023000; g) Phvul.001G014900, Glyma.14G075500; h) Phvul.006G191200; Glyma.13G245600; i) Phvul.008G013100, Glyma.18G289000. From top to bottom each panel includes: drawing of the gene model or of a different gene model for each species (not drawn to scale) with arrows indicating the position of the primers used for RT-PCR reactions and dotted lines indicating the splicing resulting in the primary transcript (above the gene model line) and the AS (below the line), the color code for different types of AS events is the same used in Fig. 1; drawings representing the amplification products expected for each transcript isoform, with its size (bp) indicated at the left; RT-PCR products resolved in 3% agarose gels, arrows indicate size (bp) of predicted fragments, the GeneRuler 1 kb Plus DNA ladder (Thermo Scientific, USA) was included for reference (third lane)