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Table 4 Proteins differentially expressed under stress conditions in strains CcI6 and CcI3

From: Genomic, transcriptomic, and proteomic approaches towards understanding the molecular mechanisms of salt tolerance in Frankia strains isolated from Casuarina trees

 

SPOT #

Acc. No

Locus Tag

Protein Name

MW (Da)

PI

NaCl

Sucrose

[C] Energy production and Conversion

CcI6

10

563,313,506

CCI6_RS13315

electron transfer flavoprotein alpha subunit apoprotein

32,842.70

5.01

↓

↓

15

563,312,797

CCI6_RS16290

NAD-dependent aldehyde dehydrogenase

54,456.60

5.40

N/C

↓

1

563,313,455

CCI6_RS13080

ATP synthase F1 subcomplex beta subunit

56,735.20

4.56

↓

N/C

8

563,314,955

CCI6_RS05745

malate dehydrogenase (NAD)

34,399.10

4.96

↑

N/C

[E] Amino acid transport and metabolism

CcI6

2

563,312,117

CCI6_RS19490

L-glutamine synthetase

53,786.20

4.97

↑

N/C

10

563,315,075

CCI6_RS05150

cysteine synthase (CysK)

32,443.60

4.95

↑

↓

[G] Carbohydrate transport and metabolism

CcI6

5

563,312,326

CCI6_RS18410

glyceraldehyde-3-phosphate dehydrogenase (NAD+)

35,515.80

5.76

↑

N/C

7

563,314,408

CCI6_RS08915

fructose-bisphosphate aldolase

36,894.20

5.35

↑

↑

CcI3

4

WP_011436076.1

FRANCCI3_RS08225

glyceraldehyde-3-phosphate dehydrogenase (NAD+)

35,515.80

5.76

↑

N/C

5

WP_011438718.1

FRANCCI3_RS22085

fructose-bisphosphate aldolase

36,894.20

5.35

↑

N/C

11

WP_011437192.1

FRANCCI3_RS14110

aldolase

38,526.7

5.3

N/C

↑

[H] Coenzyme metabolism

CcI6

3

563,314,085

CCI6_RS10325

methionine adenosyltransferase

42,893.60

5.05

↑

N/C

9

563,313,788

CCI6_RS11580

pyridoxal phosphate synthase yaaD subunit

32,577.80

5.34

↑

↑

CcI3

9

WP_011437570.1

FRANCCI3_RS16030

6,7-dimethyl-8-ribityllumazine synthase

16,137.8

5.5

↑

N/C

[I] Lipid transport and metabolism

CcI3

3

WP_011438063

 

acetyl-CoA acetyltransferase

39,714.5

5.3

↑

N/C

[J] Translation

CcI3

8

WP_011437955.1

FRANCCI3_RS18065

ribosome-recycling factor

20,830.7

5.4

↑

↑

[K] Transcription

CcI6

3

563,314,632

CCI6_RS07960

DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

44,399.30

5.10

↑

N/C

12

563,312,999

CCI6_RS15595

RNA polymerase, sigma 24 factor

29,218.90

5.49

↓

N/C

13

563,314,238

CCI6_RS09105

Transcriptional regulator Crp/Fnr

51,393.10

4.97

↓

N/C

CcI3

14

WP_011437589.1

FRANCCI3_RS16125

XRE family transcriptional regulator

17,993.4

5.7

↑

N/C

[M] Cell wall/membrane/envelop biogenesis

CcI6

6

563,313,716

CCI6_RS12035

UDP-glucose pyrophosphorylase

34,856.10

5.04

↑

↑

11

563,315,562

CCI6_RS03270

Nucleoside-diphosphate-sugar pyrophosphorylase family protein

31,492.90

4.92

↑

N/C

[O] Post-translational modification, protein turnover, chaperone functions

CcI6

2

563,314,374

CCI6_RS08745

chaperonin GroEL

56,735.20

4.72

↑

N/C

14

563,311,297

CCI6_RS22940

ATP-dependent Clp protease proteolytic subunit ClpP

23,039.30

4.79

↑

↑

[R] General Functional Prediction only

CcI6

16

563,312,796

CCI6_RS16285

Zn-dependent alcohol dehydrogenase

35,062.50

5.65

N/C

↓

CcI3

7

WP_011434678.1

FRANCCI3_RS01050

FMN reductase

21,222.1

5.3

↑

↑

Not assigned to COG categories

CcI6

4

563,313,603

CCI6_RS12255

Nitroreductase

37,351.40

4.90

↑

↑

12

563,313,563

CCI6_RS12810

proteasome endopeptidase complex

28,833.80

5.11

↑

N/C

  1. The identified proteins were classified by COG functional categories. More than one protein per spot has been identified for some spots. Upregulated proteins are shown by the upward pointing arrow (↑) whereas downregulated proteins are shown by the downward pointing arrow (↓). No change (N/C) indicates that a spot was not picked for that particular condition because it showed similar intensity as the control