Fig. 4

Fungal secondary metabolisms. a Comparison of the core genes involved in secondary metabolisms between C. cicadae (CCAD) and other closely-related fungi. ISF, I. fumosorosea; CCM, C. militaris; CCO, C. confragosa (anamorph: Lecanicillium lecanii); BBO, B. brongniartii; BBA, C. bassiana. b Venn diagram analysis of the secondary metabolic gene clusters between C. cicadae and other fungi. c Differential expression of the core secondary metabolic genes in C. cicadae at different developmental stages. PCA plotting based on metabolomic data obtained from the positive (d) and negative ion mode (e) of LC-MS analysis. There were five independent repeats for each sample. Myc, mycelia grown in SDB for seven days; Pri, primordia harvested from the mycosed tussah silkworm pupae 13 days post injection; Str, stroma harvested from the mycosed silkworm pupae 22 days post injection