Table 2 PCR primers used in this study
From: Isolation of ripening-related genes from ethylene/1-MCP treated papaya through RNA-seq
Primer | Sequence (5´–3´) |
|---|---|
EIF-F | AGGCAGGCAAGAGAAGAT |
EIF-R | TTCATACCGAGTAGCGATTC |
XTH30-F | AGCGTCCGTCTCCTCCTC |
XTH30-R | TCGTCGTGTGTCTTCTCAAATAC |
GLU-B-F | ACTGGATGCTAAGTGGTATGAAC |
GLU-B-R | GATGTCTCTCTTGGGATTCTTGG |
PMIS-F | CACCACCTCCGCCATCTC |
PMIS-R | CGCCCTGAACTCCACTTCC |
PL-F | CTCTAACTCCCATTTCACCAAACAC |
PL-R | ACCTACACCTCGGCATCCTC |
EGase-F | CTAAGCCACGCCAATAAGGTT |
EGase-R | GGATGAGCCGCAACTGATG |
EXPA-F | CGTGAAGGTGAGCGTTAAGG |
EXPA-R | TGACCGAACTGCCAATTAGC |
CHY-B-F | CTCTCCGCCGCCATTACC |
CHY-B -R | TCCTCCAACACGAAGCAGAC |
CHLM-F | GAACCACCCTCGTCGTCTC |
CHLM-R | TCCTTCACCACTTCCTTGTCC |
FER1-F | AGATGGGAACAATGACC |
FER1-R | TTACCGACCAACCTCA |
AIR3-F | GAAGAAGCATCAGAACTCGTCAAG |
AIR3-R | GAGAGAGCCAGAGGGAATTATACC |
CPSP-F | GGGAGAGAAGGGTTACATTAGGATG |
CPSP-R | TAGGATAGAGTGGAGGTTCAGTAGG |
SERPIN-F | CGGTGCCTACGATGGTTTCAAAGTT |
SERPIN-R | GAACCCAGAACCAGATCCTAACTTC |
HAB1-F | CGAAGGCGGATTCTACTGTG |
HAB1-R | GAAGAGCAAGCATTGAGAGGTAA |
GH3.1-F | CGAGTGTTACTTTGGCTTGAATCT |
GH3.1-R | TGGGCGAATTGGGTGAGT |
ERS-F | GGAGGTCACATTTGGATA |
ERS-R | TGGTTTATGCCTGGTTAG |
EBF2-F | ATCAGCAGCAGCACAGTAGA |
EBF2-R | GCCAATACCTGGAAGGGACATA |
NAC-F | TCAGTCTCGTAAGCAATCCAA |
NAC-R | TGTGGTTGTGGCATTTCTTCC |
SAG-F | TGTGGTTCTCATACGGTCTGCT |
SAG-R | CAAAGGTTCCGGCAGCTTCTTG |