Fig. 4

Modules of co-hydroxymethylated loci in the developing human brain. a Heat-map representing the correlation between module eigenvalues (ME) and the samples traits of fetal age (DPC) and sex. Each row represents a module, as indicated on the y-axis, and each column a trait. As shown in the color scale bar, strong positive correlation is indicated by dark red, strong negative correlation is indicated by dark green, and white indicates no correlation. Each cell contains the corresponding correlation and P-value given in parentheses (see also Additional file 1: Table S15). b Shown is the association between the ME and DPC for the top-ranked co-hydroxymethylated modules associated with brain development. The ‘brown’ module is the most positively associated with human fetal brain development (corr = 0.60, P = 3E-08). The ‘blue’ module is the most negatively associated with human brain development (corr = −0.48, P = 3E-05). c Heat-map of the top 1000 probes ranked by module membership in the ‘brown’ and ‘blue’ modules, showing coordinated changes in 5hmC across brain development. Color corresponds to the level of 5hmC at each probe