Fig. 1

Oocyst output and changes in intensity of E. falciformis infection in mouse. Oocyst counts in naïve and challenge infection are shown for three different mouse strains. For infection of naïve NMRI 150 oocysts were used, for challenge infection 1500 oocysts. For C57BL/6 and Rag1 ^−/− mice 10 oocysts were used in each infection. a Overall output of shed oocysts and (b) shedding kinetics are depicted. c RT-qPCR data of E. falciformis 18S in NMRI mice displays an increase in parasite mRNA over the course of infection. Significantly less parasite 18S transcripts (normalized against host transcripts of house-keeping genes) were detected in challenge infected mice. Formulas and prediction lines are given for linear models. d The percentage of parasite mRNA detected by RNA-seq increases during infection (shown for NMRI). More mRNA is detected in naïve mice compared to challenge infected mice. Sporozoites and oocysts contained ~100% parasite material