Dual transcriptome of the immediate neutrophil and Candida albicans interplay

BMC Genomics

Table 2 Cytokine secretion of neutrophils infected with C. albicans yeasts and hyphae

Cytokine	Secretion	Gene	Expression (RPKM)			h60’/y60’	FC [log2]		DEG
Cytokine	Secretion	Gene	Control	60 min y	60 min h	h60’/y60’	60 min y	60 min h	DEG
MIP-1β	UP-h	CCL4	54.4	171.8	194.5	1.1	2.20	2.35	yes
IL-1β	UP-h	IL1B	242.3	717.1	960.8	1.3	2.11	2.50	yes
IL-8	UP-h	IL8	9325.5	20,837.7	28,971.6	1.4	1.68	2.13	yes
M-CSF	UP-h	CSF1	10.6	11.8	13.6	1.2	0.68	0.86	no
TNFα	UP	TNF	30.6	39.6	30.6	0.8	0.90	0.50	no
Gro-α	n.s.	CXCL1	336.2	643.0	674.5	1.0	1.47	1.52	yes
IL-1α	n.s.	IL1A	2.6	33.3	37.7	1.1	4.24	4.39	yes
IL-1 ra	n.s.	IL1RN	225.0	486.0	524.3	1.1	1.65	1.74	yes
IL-18	n.s.	IL18	0.8	0.4	0.4	1.0	−0.34	−0.36	no
MIF	n.s.	MIF	0.8	0.4	0.3	0.7	−0.41	−0.91	no

Neutrophils were infected with dead C. albicans yeast or hypha cells and secreted cytokines were detected by Bio-Plex® at 18 h p.i.. Cytokines were either induced similarly with both (UP), secreted more in the presence of hyphae (UP-h), or not secreted (n.s.). Cytokine gene transcript levels (by RPKM) were analyzed after 60 min of stimulation, transcript levels in the infection with yeast and hyphae were related (h60’/y60’). Cytokine gene fold changes of expression (FC) are displayed after 60 min of cocultivation of neutrophils with C. albicans yeasts or hyphae. Statistically significant DEGs are pointed out

ISSN: 1471-2164