Transcriptome profiling of antiviral immune and dietary fatty acid dependent responses of Atlantic salmon macrophage-like cells

Eslamloo, Khalil; Xue, Xi; Hall, Jennifer R.; Smith, Nicole C.; Caballero-Solares, Albert; Parrish, Christopher C.; Taylor, Richard G.; Rise, Matthew L.

doi:10.1186/s12864-017-4099-2

Table 2 Lipid class and fatty acid composition of salmon head kidney leukocytes (HKLs) in different dietary groups

From: Transcriptome profiling of antiviral immune and dietary fatty acid dependent responses of Atlantic salmon macrophage-like cells

Lipid class %	FO7	FO5	p value
Hydrocarbons	0.76 ± 0.104	0.43 ± 0.177	0.110
Triacylglycerols	0.83 ± 0.182	1.94 ± 0.641	0.124
Free fatty acids	0.58 ± 0.162	1.99 ± 0.442	0.012
Sterols	11.38 ± 0.318	12.51 ± 0.371	0.032
AMPL^a	5.71 ± 0.787	6.06 ± 0.909	0.777
Phospholipids	80.74 ± 0.816	76.80 ± 1.695	0.044
Fatty acids %^b
14:0	1.17 ± 0.027	0.97 ± 0.030	< 0.0001
15:0	0.24 ± 0.003	0.22 ± 0.005	0.001
16:0	18.28 ± 0.189	17.96 ± 0.245	0.314
16:1n-7	0.77 ± 0.032	0.73 ± 0.050	0.473
17:0	0.21 ± 0.004	0.21 ± 0.005	0.200
16:4n-1	2.19 ± 0.289	2.08 ± 0.356	0.814
18:0	6.36 ± 0.110	6.45 ± 0.149	0.621
18:1n-9	17.08 ± 0.337	17.81 ± 0.349	0.151
18:1n-7	3.15 ± 0.031	3.16 ± 0.043	0.840
18:2n-6	4.58 ± 0.084	4.93 ± 0.142	0.041
18:3n-6	0.20 ± 0.005	0.27 ± 0.011	< 0.0001
18:3n-3	0.76 ± 0.025	0.79 ± 0.038	0.568
18:4n-3	0.26 ± 0.008	0.30 ± 0.023	0.073
20:1n-9	1.14 ± 0.037	0.98 ± 0.036	0.005
20:2n-6	0.63 ± 0.020	0.63 ± 0.030	0.922
20:3n-6	1.57 ± 0.046	1.88 ± 0.078	0.003
20:4n-6	5.23 ± 0.152	5.67 ± 0.190	0.080
20:4n-3	0.56 ± 0.017	0.55 ± 0.018	0.739
20:5n-3	6.37 ± 0.150	5.77 ± 0.131	0.008
22:1n-11(13)	0.24 ± 0.024	0.32 ± 0.062	0.232
22:1n-9	0.27 ± 0.051	0.46 ± 0.112	0.131
22:5n-3	0.74 ± 0.027	0.69 ± 0.023	0.245
22:6n-3	23.74 ± 0.448	23.00 ± 0.473	0.271
24:1	0.72 ± 0.024	0.74 ± 0.029	0.450
Bacterial	1.14 ± 0.047	1.05 ± 0.049	0.196
Σ SFA^c	26.41 ± 0.298	25.94 ± 0.399	0.346
Σ MUFA^d	24.48 ± 0.397	25.25 ± 0.482	0.229
Σ PUFA^e	48.53 ± 0.594	48.28 ± 0.624	0.774
Σ LC n-3^f	31.58 ± 0.551	30.16 ± 0.531	0.080
Σ LC n-6	7.68 ± 0.171	8.47 ± 0.226	0.012
LCn-6/ LCn-3	0.24 ± 0.005	0.28 ± 0.007	0.001
P/S	1.84 ± 0.040	1.87 ± 0.047	0.662
Σ n-3	33.43 ± 0.583	32.13 ± 0.449	0.098
DHA/EPA ratio	3.74 ± 0.087	4.00 ± 0.102	0.068

Values are mean ± SE. Bold p values indicate a significant (p < 0.05) difference between groups
^aAcetone mobile polar lipids
^bData are expressed as area percentage of identified FAME (fatty acid methyl ester), for fatty acids that were present at ≥0.2% of the total
^cSaturated fatty acid
^dMonounsaturated fatty acid
^ePolyunsaturated fatty acid
^fLong chain n-3
Other fatty acids present at <0.2%: 15:0, i16:0, 16:1n-5, i17:0, ai17:0, 16:2n-4, 17:1, 18:2n-4, 18:3n-4, 20:0, 20:3n-3, 22:0, 22:5n-6

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